論文 - 詳細
| RRC ID | 39954 |
|---|---|
| 著者 | Dohmoto K, Hojo S, Fujita J, Yang Y, Ueda Y, Bandoh S, Yamaji Y, Ohtsuki Y, Dobashi N, Ishida T, Takahara J. |
| タイトル | The role of caspase 3 in producing cytokeratin 19 fragment (CYFRA21-1) in human lung cancer cell lines. |
| ジャーナル | Int J Cancer |
| Abstract |
The CYFRA 21-1 assay, which detects cytokeratin 19 (CK19) fragment, is widely used as a tumor marker for lung cancer, particularly non-small cell lung cancer. However, the reason that some lung cancer cell lines release CYFRA 21-1 in culture supernatants and others do not remains unclear. We hypothesized that the release of CYFRA 21-1 might be related to the expression of CK19 and caspase 3. In order to prove this, the quantities of mRNA for CK19 were evaluated by the competitive reverse transcriptase-polymerase chain reaction (RT-PCR). CK19 protein synthesis was also evaluated by Western blotting and immunohistochemistry, and the levels of CYFRA 21-1 in the culture supernatant were measured by an immunoradiometric assay. The expression of mRNA for caspase 3 was evaluated by the RT-PCR, and caspase 3 protein synthesis was also evaluated by immunohistochemistry. In 13 lung cancer cell lines, the amounts of mRNA for CK19 correlated with the levels of CYFRA 21-1 in culture supernatants, results of Western blotting for CK19, and positivities of immunohistochemistry for CK19. In 5 cell lines that produced a significant amount of CYFRA 21-1, the level of CYFRA 21-1 correlated with the positivity of RT-PCR for caspase 3 and immunohistochmistry for caspase 3. This suggests that caspase 3 played a role in the formation of CYFRA 21-1. In addition, the specific inhibitor of caspase 3 significantly inhibited the release of CYFRA 21-1 in culture supernatants. In conclusion, we demonstrate that caspase 3, which cleaves several intermediate filaments and carries out cell apoptosis, played an important role in producing CYFRA 21-1 in human lung cancer cell lines. |
| 巻・号 | 91(4) |
| ページ | 468-73 |
| 公開日 | 2001-2-15 |
| DOI | 10.1002/1097-0215(200002)9999:9999<::aid-ijc1082>3.0.co;2-t |
| PII | 10.1002/1097-0215(200002)9999:9999<::AID-IJC1082>3.0.CO;2-T |
| PMID | 11251967 |
| MeSH | Animals Antibodies, Monoclonal / metabolism Antigens, Neoplasm / biosynthesis* Apoptosis Biomarkers, Tumor Blotting, Western Caspase 3 Caspase 6 Caspases / biosynthesis Caspases / physiology* Electrophoresis, Polyacrylamide Gel Humans Immunohistochemistry Keratin-19 Keratins / biosynthesis* Lung Neoplasms / metabolism* Mice Models, Genetic RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Time Factors Tumor Cells, Cultured |
| IF | 5.145 |
| 引用数 | 51 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LC-2/ad(RCB0440) Lu-135(RCB0468) Lu-165(RCB1184) |