| RRC ID |
39968
|
| Author |
Abe T, Tamiya T, Ono Y, Salker AH, Akiyama K, Ohmoto T.
|
| Title |
Accumulation of cell cycle regulatory proteins, p21 and p27, induced after hyperthermia in human glioma cells.
|
| Journal |
Int J Hyperthermia
|
| Abstract |
It was investigated whether there was a relationship between p53 p21 and p27 induction pathways in the cellular response of glioma cells to hyperthermia. Two glioma cell lines were employed. A-172 cells had the wild-type of p53, and U251 cells had the mutant-type of p53. An adenovirus harbouring wild-type p53 was also used for the overexpression. The protein induction by hyperthermia was monitored by Western blot analysis. In U251 cells, the expression of wild-type p53 and hyperthermia had an additional cytotoxic effect, but did not affect A-172 cells. Significant p21 accumulation by hyperthermia was recognized in A-172 cells, and was also recognized in p53-transduced U251 cells. On the other hand, the accumulation of p27 by hyperthermia was not seen in A-172 or U251 cells, and the exogenous expression of p53 did not affect the accumulation of p27 by hyperthermia in U251 cells. These findings suggest that the p53-p21 pathway is involved in the signal transduction after hyperthermia, rather than the p27 pathway.
|
| Volume |
17(6)
|
| Pages |
499-507
|
| Published |
2001-1-1
|
| DOI |
10.1080/02656730110070219
|
| PMID |
11719966
|
| MeSH |
Adenoviridae / genetics
Cell Cycle Proteins / metabolism*
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinase Inhibitor p27
Cyclins / metabolism*
Genes, p53
Genetic Vectors
Glioma / genetics
Glioma / metabolism*
Glioma / therapy*
Humans
Hyperthermia, Induced*
Lac Operon
Signal Transduction
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / metabolism
Tumor Suppressor Proteins / metabolism*
|
| IF |
3.574
|
| Times Cited |
8
|
|
WOS Category
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
U251(RCB0461) |
