| RRC ID |
39978
|
| Author |
Randlett O, Wee CL, Naumann EA, Nnaemeka O, Schoppik D, Fitzgerald JE, Portugues R, Lacoste AM, Riegler C, Engert F, Schier AF.
|
| Title |
Whole-brain activity mapping onto a zebrafish brain atlas.
|
| Journal |
Nat Methods
|
| Abstract |
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors.
|
| Volume |
12(11)
|
| Pages |
1039-46
|
| Published |
2015-11-1
|
| DOI |
10.1038/nmeth.3581
|
| PMID |
26778924
|
| PMC |
PMC4710481
|
| MeSH |
Algorithms
Animals
Automation
Behavior, Animal
Brain / metabolism*
Brain / physiology
Brain Mapping / methods
Calcium / chemistry
Extracellular Signal-Regulated MAP Kinases / metabolism*
Image Processing, Computer-Assisted / methods*
Immunohistochemistry
Microscopy, Confocal
Neurites / metabolism*
Neurons / metabolism
Neurons / physiology
Phosphorylation
Principal Component Analysis
Reproducibility of Results
Software
Swimming
Zebrafish
|
| IF |
30.822
|
| Times Cited |
140
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
|
| Resource |
| Zebrafish |
Tg（isl1:GFP)/rw0
Tg(gad1b:GFP) |
