Reference - Detail
| RRC ID | 39978 |
|---|---|
| Author | Randlett O, Wee CL, Naumann EA, Nnaemeka O, Schoppik D, Fitzgerald JE, Portugues R, Lacoste AM, Riegler C, Engert F, Schier AF. |
| Title | Whole-brain activity mapping onto a zebrafish brain atlas. |
| Journal | Nat Methods |
| Abstract |
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors. |
| Volume | 12(11) |
| Pages | 1039-46 |
| Published | 2015-11-1 |
| DOI | 10.1038/nmeth.3581 |
| PMID | 26778924 |
| PMC | PMC4710481 |
| MeSH | Algorithms Animals Automation Behavior, Animal Brain / metabolism* Brain / physiology Brain Mapping / methods Calcium / chemistry Extracellular Signal-Regulated MAP Kinases / metabolism* Image Processing, Computer-Assisted / methods* Immunohistochemistry Microscopy, Confocal Neurites / metabolism* Neurons / metabolism Neurons / physiology Phosphorylation Principal Component Analysis Reproducibility of Results Software Swimming Zebrafish |
| IF | 30.822 |
| Times Cited | 140 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 20 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Zebrafish | Tg（isl1:GFP)/rw0 Tg(gad1b:GFP) |