RRC ID 40001
Author Kyung T, Lee S, Kim JE, Cho T, Park H, Jeong YM, Kim D, Shin A, Kim S, Baek J, Kim J, Kim NY, Woo D, Chae S, Kim CH, Shin HS, Han YM, Kim D, Heo WD.
Title Optogenetic control of endogenous Ca(2+) channels in vivo.
Journal Nat. Biotechnol.
Abstract Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we present OptoSTIM1, an optogenetic tool for manipulating intracellular Ca(2+) levels through activation of Ca(2+)-selective endogenous Ca(2+) release-activated Ca(2+) (CRAC) channels. Using OptoSTIM1, which combines a plant photoreceptor and the CRAC channel regulator STIM1 (ref. 4), we quantitatively and qualitatively controlled intracellular Ca(2+) levels in various biological systems, including zebrafish embryos and human embryonic stem cells. We demonstrate that activating OptoSTIM1 in the CA1 hippocampal region of mice selectively reinforced contextual memory formation. The broad utility of OptoSTIM1 will expand our mechanistic understanding of numerous Ca(2+)-associated processes and facilitate screening for drug candidates that antagonize Ca(2+) signals.
Volume 33(10)
Pages 1092-6
Published 2015-10
DOI 10.1038/nbt.3350
PII nbt.3350
PMID 26368050
MeSH Animals Calcium / metabolism* Calcium Channels / genetics* Calcium Signaling / genetics* Embryonic Stem Cells / cytology Embryonic Stem Cells / physiology* Genetic Engineering / methods Hippocampus / cytology Hippocampus / physiology* Humans Mice Optogenetics / methods*
IF 35.724
Times Cited 15
Zebrafish Tg(huC:GVP)