| RRC ID |
40010
|
| 著者 |
Kotani Y, Morito D, Yamazaki S, Ogino K, Kawakami K, Takashima S, Hirata H, Nagata K.
|
| タイトル |
Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Mysterin (also known as RNF213) is a huge intracellular protein with two AAA+ ATPase modules and a RING finger ubiquitin ligase domain. Mysterin was originally isolated as a significant risk factor for the cryptogenic cerebrovascular disorder moyamoya disease, and was found to be involved in physiological angiogenesis in zebrafish. However, the function and the physiological significance of mysterin in other than blood vessels remain largely unknown, although mysterin is ubiquitously expressed in animal tissues. In this study, we performed antisense-mediated suppression of a mysterin orthologue in zebrafish larvae and revealed that mysterin-deficient larvae showed significant reduction in fast myofibrils and immature projection of primary motoneurons, leading to severe motor deficits. Fast muscle-specific restoration of mysterin expression cancelled these phenotypes, and interestingly both AAA+ ATPase and ubiquitin ligase activities of mysterin were indispensable for proper fast muscle formation, demonstrating an essential role of mysterin and its enzymatic activities in the neuromuscular regulation in zebrafish.
|
| 巻・号 |
5
|
| ページ |
16161
|
| 公開日 |
2015-11-4
|
| DOI |
10.1038/srep16161
|
| PII |
srep16161
|
| PMID |
26530008
|
| PMC |
PMC4632019
|
| MeSH |
Animals
Animals, Genetically Modified / growth & development
Animals, Genetically Modified / metabolism
Humans
Immunohistochemistry
Larva / metabolism
Microscopy, Electron
Motor Activity
Motor Neurons / metabolism
Muscle Fibers, Skeletal / metabolism
Muscle Fibers, Skeletal / pathology
Neovascularization, Physiologic
Oligonucleotides, Antisense / metabolism
RING Finger Domains
Ubiquitin-Protein Ligases / antagonists & inhibitors
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
Zebrafish / growth & development
Zebrafish / metabolism*
Zebrafish Proteins / antagonists & inhibitors
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
| IF |
3.998
|
| 引用数 |
11
|
|
WOS 分野
|
MULTIDISCIPLINARY SCIENCES
|
| リソース情報 |
| ゼブラフィッシュ |
gSA2AzGFF586A?
gSA2AzGFF591A ?
UASRFP1A? |
