論文 - 詳細
| RRC ID | 40019 |
|---|---|
| 著者 | Xiao Y, Faucherre A, Pola-Morell L, Heddleston JM, Liu TL, Chew TL, Sato F, Sehara-Fujisawa A, Kawakami K, López-Schier H. |
| タイトル | High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish. |
| ジャーナル | Dis Model Mech |
| Abstract |
Neural damage is a devastating outcome of physical trauma. The glia are one of the main effectors of neuronal repair in the nervous system, but the dynamic interactions between peripheral neurons and Schwann cells during injury and regeneration remain incompletely characterized. Here, we combine laser microsurgery, genetic analysis, high-resolution intravital imaging and lattice light-sheet microscopy to study the interaction between Schwann cells and sensory neurons in a zebrafish model of neurotrauma. We found that chronic denervation by neuronal ablation leads to Schwann-cell death, whereas acute denervation by axonal severing does not affect the overall complexity and architecture of the glia. Neuronal-circuit regeneration begins when Schwann cells extend bridging processes to close the injury gap. Regenerating axons grow faster and directionally after the physiological clearing of distal debris by the Schwann cells. This might facilitate circuit repair by ensuring that axons are guided through unoccupied spaces within bands of Büngner towards their original peripheral target. Accordingly, in the absence of Schwann cells, regenerating axons are misrouted, impairing the re-innervation of sensory organs. Our results indicate that regenerating axons use haptotaxis as a directional cue during the reconstitution of a neural circuit. These findings have implications for therapies aimed at neurorepair, which will benefit from preserving the architecture of the peripheral glia during periods of denervation. |
| 巻・号 | 8(6) |
| ページ | 553-64 |
| 公開日 | 2015-6-1 |
| DOI | 10.1242/dmm.018184 |
| PII | dmm.018184 |
| PMID | 26035865 |
| PMC | PMC4457030 |
| MeSH | Animals Animals, Genetically Modified Axons / metabolism* Cell Communication* Chronic Disease Claudins / metabolism Denervation Homozygote Lasers Molecular Imaging / methods* Mutagenesis, Insertional / genetics Myelin-Associated Glycoprotein / metabolism Nerve Regeneration* Neuroglia / metabolism* Peripheral Nerve Injuries / pathology* Phenotype Schwann Cells / metabolism Zebrafish / physiology* Zebrafish Proteins / genetics |
| IF | 4.651 |
| 引用数 | 20 |
| WOS 分野 | PATHOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | gSAGFF202A? |