Reference - Detail
RRC ID | 4005 |
---|---|
Author | Maruyama N, Okuda E, Tatsuhara M, Utsumi S. |
Title | Aggregation of proteins having Golgi apparatus sorting determinant induces large globular structures derived from the endoplasmic reticulum in plant seed cells. |
Journal | FEBS Lett |
Abstract |
Endoplasmic reticulum (ER)-derived compartments are found in many plant species. Although it has been assumed that aggregation induces formation of the ER-derived compartments in plant seed cells, the effect of aggregation on the trafficking from the ER to the Golgi has not yet been elucidated. In this study, we used an aggregated type of red fluorescent protein (DsRED) to investigate the effect of aggregation on sorting in seed cells. DsRED fused to the Golgi sorting determinant was found mainly in large globular structures derived from the ER where ER-resident proteins were excluded. These results indicate that aggregation of the Golgi protein blocks transport from the ER to the Golgi. |
Volume | 582(11) |
Pages | 1599-606 |
Published | 2008-5-14 |
DOI | 10.1016/j.febslet.2008.04.008 |
PII | S0014-5793(08)00322-0 |
PMID | 18423406 |
MeSH | Animals Anthozoa Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis / ultrastructure Brefeldin A / pharmacology Endoplasmic Reticulum / drug effects Endoplasmic Reticulum / metabolism* Golgi Apparatus / drug effects Golgi Apparatus / metabolism* Luminescent Proteins / chemistry Luminescent Proteins / genetics Luminescent Proteins / metabolism Mutation Pentosyltransferases / chemistry Pentosyltransferases / genetics Pentosyltransferases / metabolism Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Plants, Genetically Modified / ultrastructure Protein Conformation Protein Folding Protein Transport Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Seeds / metabolism Seeds / ultrastructure |
IF | 3.057 |
Times Cited | 4 |
WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
Resource | |
Arabidopsis / Cultured plant cells, genes | pda08639 pda12219 pda00289 |