Reference - Detail
| RRC ID | 40094 |
|---|---|
| Author | Winkelströter LK, Dolan SK, Fernanda Dos Reis T, Bom VL, Alves de Castro P, Hagiwara D, Alowni R, Jones GW, Doyle S, Brown NA, Goldman GH. |
| Title | Systematic Global Analysis of Genes Encoding Protein Phosphatases in Aspergillus fumigatus. |
| Journal | G3 (Bethesda) |
| Abstract |
Aspergillus fumigatus is a fungal pathogen that causes several invasive and noninvasive diseases named aspergillosis. This disease is generally regarded as multifactorial, considering that several pathogenicity determinants are present during the establishment of this illness. It is necessary to obtain an increased knowledge of how, and which, A. fumigatus signal transduction pathways are engaged in the regulation of these processes. Protein phosphatases are essential to several signal transduction pathways. We identified 32 phosphatase catalytic subunit-encoding genes in A. fumigatus, of which we were able to construct 24 viable deletion mutants. The role of nine phosphatase mutants in the HOG (high osmolarity glycerol response) pathway was evaluated by measuring phosphorylation of the p38 MAPK (SakA) and expression of osmo-dependent genes. We were also able to identify 11 phosphatases involved in iron assimilation, six that are related to gliotoxin resistance, and three implicated in gliotoxin production. These results present the creation of a fundamental resource for the study of signaling in A. fumigatus and its implications in the regulation of pathogenicity determinants and virulence in this important pathogen. |
| Volume | 5(7) |
| Pages | 1525-39 |
| Published | 2015-5-5 |
| DOI | 10.1534/g3.115.016766 |
| PII | g3.115.016766 |
| PMID | 25943523 |
| PMC | PMC4502386 |
| MeSH | Aspergillus fumigatus / enzymology* Catalytic Domain Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Fungal Proteins / classification Fungal Proteins / genetics* Fungal Proteins / metabolism Gene Expression Regulation, Fungal Gliotoxin / analysis Gliotoxin / metabolism Mutation Phenotype Phosphoprotein Phosphatases / classification Phosphoprotein Phosphatases / genetics* Phosphoprotein Phosphatases / metabolism Phosphorylation Phylogeny Siderophores / analysis Signal Transduction Tandem Mass Spectrometry Virulence / genetics p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 2.781 |
| Times Cited | 20 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Blogs |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
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| Pathogenic microorganisms | |