RRC ID 40098
Author Matsuyama A, Shirai A, Yoshida M.
Title A novel series of vectors for chromosomal integration in fission yeast.
Journal Biochem Biophys Res Commun
Abstract A series of fission yeast targeting vectors that can be used for wild-type strains having no selectable markers have been designed. The functions of one of three marker genes, lys1(+), arg1(+), and his3(+), involved in amino acid synthesis, are impaired by integration of the fragments generated by restriction enzyme digestion of the plasmids. Successful integration of the fragments into the targeted loci can be readily verified by their requirement for amino acids, or by the PCR diagnostic analysis. Since these selection markers are not used commonly in fission yeast, these plasmids are likely to facilitate studies that require the co-expression of genes such as co-localization and co-immunoprecipitation experiments, by employing them in combination with most of the previously reported markers.
Volume 374(2)
Pages 315-9
Published 2008-9-19
DOI 10.1016/j.bbrc.2008.07.015
PII S0006-291X(08)01343-0
PMID 18634753
MeSH Arginine / biosynthesis Arginine / genetics Chromosomes, Fungal / genetics* Gene Targeting / methods* Genetic Markers Genetic Vectors* Histidine / biosynthesis Histidine / genetics Immunoprecipitation Lysine / biosynthesis Lysine / genetics Plasmids* Schizosaccharomyces / genetics*
IF 2.985
Times Cited 10
DNA material pHIS3K (RDB06561) pLYS1K (RDB06553) pARG1K (RDB06557) pHIS3U (RDB06560) pLYS1U (RDB06552) pARG1U (RDB06556) pHIS3H (RDB06562) pLYS1H (RDB06554) pARG1H (RDB06558) pHIS3B (RDB06563) pLYS1B (RDB06555) pARG1B (RDB06559).