論文 - 詳細
| RRC ID | 4012 |
|---|---|
| 著者 | Shoji T, Hashimoto T. |
| タイトル | Why does anatabine, but not nicotine, accumulate in jasmonate-elicited cultured tobacco BY-2 cells? |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Suspension-cultured cells of Nicotiana tabacum cv. Bright Yellow-2 (BY-2) grow rapidly in a highly homogenous population and still exhibit the general behavior of plant cells, and thus are often used as model systems in several areas of plant molecular and cellular biology, including secondary metabolism. While the parental tobacco variety synthesizes nicotine as a major alkaloid, the cultured tobacco cells mainly produce a related alkaloid anatabine, instead of nicotine, when elicited with jasmonates. We report here that cultured BY-2 cells scarcely express N-methylputrescine oxidase (MPO) genes even after jasmonate elicitation. MPO is the second enzyme in the biosynthetic pathway that supplies the pyrrolidine moiety of nicotine and nornicotine, but is predicted to be dispensable for the biosynthesis of anatabine, anabasine and anatalline, which do not contain the pyrrolidine moiety. When MPO was overexpressed in tobacco BY-2 cells, nicotine synthesis was dramatically enhanced while anatabine formation was effectively suppressed. As a complementary approach, we suppressed MPO expression by RNA interference in tobacco hairy roots that normally accumulate nicotine. In the MPO-suppressed roots, the contents of anatabine, anabasine and anatalline, as well as N-methylputrescine and putrescine, markedly increased to compensate for suppressed formation of nicotine and nornicotine. These results identify the transcriptional regulation of MPO as a critical rate-limiting step that restricts nicotine formation in cultured tobacco BY-2 cells. |
| 巻・号 | 49(8) |
| ページ | 1209-16 |
| 公開日 | 2008-8-1 |
| DOI | 10.1093/pcp/pcn096 |
| PII | pcn096 |
| PMID | 18567891 |
| MeSH | Alkaloids / biosynthesis* Alkaloids / chemistry Cells, Cultured Cyclopentanes / pharmacology* Down-Regulation Gene Expression Regulation, Plant / drug effects Molecular Structure Nicotine / biosynthesis* Nicotine / chemistry Oxidoreductases / metabolism Oxylipins / pharmacology* Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / metabolism Pyridines / chemistry RNA Interference Tobacco / cytology* Tobacco / drug effects* |
| IF | 4.062 |
| 引用数 | 25 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00035 |