| RRC ID |
4017
|
| Author |
Kamada-Nobusada T, Hayashi M, Fukazawa M, Sakakibara H, Nishimura M.
|
| Title |
A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana.
|
| Journal |
Plant Cell Physiol
|
| Abstract |
We characterized three Arabidopsis polyamine oxidase genes, AtPAO2, AtPAO3 and AtPAO4. Transient expression of these genes as monomeric red fluorescent protein fusion proteins in Arabidopsis root cells revealed that all are peroxisomal proteins. Quantitative analysis of their transcripts in various organs suggested that AtPAO4 is the major isoform in root peroxisomes. Analysis of recombinant AtPAO4 protein indicated that it is a flavoprotein that catalyzed the oxidative conversion of spermine to spermidine. AtPAO4-deficient mutants established by using T-DNA insertion and RNA interference techniques had markedly increased spermine and decreased spermidine levels in the roots. These results suggest that AtPAO4 is a root peroxisomal polyamine oxidase that participates in polyamine catabolism. Microarray analysis showed that AtPAO4 deficiency induced alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis.
|
| Volume |
49(9)
|
| Pages |
1272-82
|
| Published |
2008-9-1
|
| DOI |
10.1093/pcp/pcn114
|
| PII |
pcn114
|
| PMID |
18703589
|
| MeSH |
Arabidopsis / enzymology*
Arabidopsis / genetics*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
DNA, Bacterial / genetics
Escherichia coli / genetics
Escherichia coli / metabolism
Gene Expression Regulation, Plant
Genes, Plant
Mutagenesis, Insertional
Oligonucleotide Array Sequence Analysis
Oxidoreductases Acting on CH-NH Group Donors / genetics
Oxidoreductases Acting on CH-NH Group Donors / metabolism*
Peroxisomes / genetics
Peroxisomes / metabolism*
Plant Roots / enzymology
Plant Roots / genetics
RNA Interference
RNA, Plant / genetics
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Spermidine / metabolism
Spermine / metabolism
Substrate Specificity
|
| IF |
4.062
|
| Times Cited |
104
|
|
WOS Category
|
PLANT SCIENCES
CELL BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda07387
pda02195
pda04373 |
