| RRC ID |
4025
|
| Author |
Nishikawa M, Hosokawa K, Ishiguro M, Minamioka H, Tamura K, Hara-Nishimura I, Takahashi Y, Shimazaki K, Imai H.
|
| Title |
Degradation of sphingoid long-chain base 1-phosphates (LCB-1Ps): functional characterization and expression of AtDPL1 encoding LCB-1P lyase involved in the dehydration stress response in Arabidopsis.
|
| Journal |
Plant Cell Physiol
|
| Abstract |
Sphingoid long-chain base (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C(16) fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants more than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.
|
| Volume |
49(11)
|
| Pages |
1758-63
|
| Published |
2008-11-1
|
| DOI |
10.1093/pcp/pcn149
|
| PII |
pcn149
|
| PMID |
18849574
|
| MeSH |
Aldehyde-Lyases / genetics
Aldehyde-Lyases / metabolism*
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
DNA, Bacterial / genetics
Dehydration / metabolism*
Endoplasmic Reticulum / metabolism
Gene Deletion
Gene Expression Regulation, Plant
Genes, Plant
Genetic Complementation Test
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Mutagenesis, Insertional
Mutation
Phosphates / metabolism*
Plant Transpiration
RNA, Plant / genetics
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics
Stress, Physiological
|
| IF |
4.062
|
| Times Cited |
25
|
|
WOS Category
|
PLANT SCIENCES
CELL BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda02078 |
