Reference - Detail
| RRC ID | 4056 |
|---|---|
| Author | An CI, Sawada A, Kawaguchi Y, Fukusaki E, Kobayashi A. |
| Title | Transient RNAi induction against endogenous genes in Arabidopsis protoplasts using in vitro-prepared double-stranded RNA. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes. |
| Volume | 69(2) |
| Pages | 415-8 |
| Published | 2005-2-1 |
| DOI | 10.1271/bbb.69.415 |
| PII | JST.JSTAGE/bbb/69.415 |
| PMID | 15725671 |
| MeSH | Arabidopsis / genetics* Cells, Cultured Gene Dosage Gene Expression Regulation, Plant Gene Silencing Protoplasts / physiology* RNA Interference* RNA, Double-Stranded / physiology* Time Factors |
| IF | 1.516 |
| Times Cited | 18 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 47 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00008 |