| RRC ID |
4056
|
| Author |
An CI, Sawada A, Kawaguchi Y, Fukusaki E, Kobayashi A.
|
| Title |
Transient RNAi induction against endogenous genes in Arabidopsis protoplasts using in vitro-prepared double-stranded RNA.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.
|
| Volume |
69(2)
|
| Pages |
415-8
|
| Published |
2005-2-1
|
| DOI |
10.1271/bbb.69.415
|
| PII |
JST.JSTAGE/bbb/69.415
|
| PMID |
15725671
|
| MeSH |
Arabidopsis / genetics*
Cells, Cultured
Gene Dosage
Gene Expression Regulation, Plant
Gene Silencing
Protoplasts / physiology*
RNA Interference*
RNA, Double-Stranded / physiology*
Time Factors
|
| IF |
1.516
|
| Times Cited |
18
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
