論文 - 詳細
| RRC ID | 4067 |
|---|---|
| 著者 | An CI, Sawada A, Fukusaki E, Kobayashi A. |
| タイトル | A transient RNA interference assay system using Arabidopsis protoplasts. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana. |
| 巻・号 | 67(12) |
| ページ | 2674-7 |
| 公開日 | 2003-12-1 |
| DOI | 10.1271/bbb.67.2674 |
| PMID | 14730153 |
| MeSH | Arabidopsis / genetics* Cells, Cultured DNA Primers / genetics Gene Expression Regulation, Plant / genetics* Genes, Reporter / genetics* Genetic Vectors / genetics* Protoplasts / metabolism RNA Interference* RNA, Double-Stranded / genetics Transfection |
| IF | 1.516 |
| 引用数 | 21 |
| WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |