論文 - 詳細
RRC ID | 4067 |
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著者 | An CI, Sawada A, Fukusaki E, Kobayashi A. |
タイトル | A transient RNA interference assay system using Arabidopsis protoplasts. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana. |
巻・号 | 67(12) |
ページ | 2674-7 |
公開日 | 2003-12-1 |
DOI | 10.1271/bbb.67.2674 |
PMID | 14730153 |
MeSH | Arabidopsis / genetics* Cells, Cultured DNA Primers / genetics Gene Expression Regulation, Plant / genetics* Genes, Reporter / genetics* Genetic Vectors / genetics* Protoplasts / metabolism RNA Interference* RNA, Double-Stranded / genetics Transfection |
IF | 1.516 |
引用数 | 21 |
WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |