RRC ID 4067
Author An CI, Sawada A, Fukusaki E, Kobayashi A.
Title A transient RNA interference assay system using Arabidopsis protoplasts.
Journal Biosci Biotechnol Biochem
Abstract Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
Volume 67(12)
Pages 2674-7
Published 2003-12-1
DOI 10.1271/bbb.67.2674
PMID 14730153
MeSH Arabidopsis / genetics* Cells, Cultured DNA Primers / genetics Gene Expression Regulation, Plant / genetics* Genes, Reporter / genetics* Genetic Vectors / genetics* Protoplasts / metabolism RNA Interference* RNA, Double-Stranded / genetics Transfection
IF 1.516
Times Cited 21
WOS Category CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes rpc00008