| Abstract |
A Gram-staining-positive, aerobic, motile and non-spore-forming actinobacteria, designated strain F10(T), was isolated from a deep-sea sediment of the western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus Nesterenkonia. Strain F10(T) shared highest 16S rRNA gene sequence similarity of 96.8 % with Nesterenkonia aethiopica DSM 17733(T), followed by Nesterenkonia xinjiangensis YIM 70097(T) (96.7 %) and Nesterenkonia alba CAAS 252(T) (96.6 %). The organism grew at 4-50 °C, at pH 7.0-12.0 and in the presence of 0-12 % (w/v) NaCl, with optimal growth occurring at 40 °C, at pH 9.0 and in the presence of 1 % (w/v) NaCl. The peptidoglycan type was A4(alpha), l-Lys-Gly-l-Glu. The polar lipid profile of strain F10(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and two unknown lipids. The isolate contained MK-9 (92 %) and MK-8 (5.8 %) as the major components of the menaquinone system, and anteiso-C17 : 0 (50.9 %) and anteiso-C15 : 0 (29.8 %) as the predominant fatty acids. The G+C content of the genomic DNA of strain F10(T) was 66.2 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain F10(T) represents a novel species of the genus Nesterenkonia for which the name Nesterenkonia alkaliphila sp. nov. is proposed. The type strain is F10(T) ( = LMG 28112(T) = CGMCC 1.12781(T) = JCM 19766(T) = MCCC 1A09946(T)).
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