論文 - 詳細
| RRC ID | 40843 |
|---|---|
| 著者 | Grosche A, Sekaran H, Pérez-Rodríguez I, Starovoytov V, Vetriani C. |
| タイトル | Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6(T), was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9° N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1-1.5 µm in length and 0.5 µm in width. Strain TB-6(T) grew between 45 and 70 °C (optimum 55-60 °C), 0 and 35 g NaCl l(-1) (optimum 20-30 g l(-1)) and pH 4.5 and 7.5 (optimum pH 5.5-6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6(T) occurred with H2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-d-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H2/CO2 (80 : 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6(T) showed that this organism branched separately from the three most closely related genera, Caminibacter , Nautilia and Lebetimonas , within the family Nautiliaceae . Strain TB-6(T) contained several unique fatty acids in comparison with other members of the family Nautiliaceae . Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae , Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6(T) ( = DSM 27783(T) = JCM 19563(T)). |
| 巻・号 | 65(Pt 4) |
| ページ | 1144-1150 |
| 公開日 | 2015-4-1 |
| DOI | 10.1099/ijs.0.000070 |
| PMID | 25604337 |
| MeSH | Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Epsilonproteobacteria / classification* Epsilonproteobacteria / genetics Epsilonproteobacteria / isolation & purification Fatty Acids / chemistry Hydrothermal Vents / microbiology* Molecular Sequence Data Nitrates / metabolism Pacific Ocean Phylogeny* RNA, Ribosomal, 16S / genetics Seawater / microbiology Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| IF | 2.415 |
| 引用数 | 7 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 19563 |