RRC ID 40909
著者 Zeng X, Zhang Z, Li X, Jebbar M, Alain K, Shao Z.
タイトル Caloranaerobacter ferrireducens sp. nov., an anaerobic, thermophilic, iron (III)-reducing bacterium isolated from deep-sea hydrothermal sulfide deposits.
ジャーナル Int J Syst Evol Microbiol
Abstract A thermophilic, anaerobic, iron-reducing bacterium (strain DY22619T) was isolated from a sulfide sample collected from an East Pacific Ocean hydrothermal field at a depth of 2901 m. Cells were Gram-stain-negative, motile rods (2-10 µm in length, 0.5 µm in width) with multiple peritrichous flagella. The strain grew at 40-70 °C inclusive (optimum 60 °C), at pH 4.5-8.5 inclusive (optimum pH 7.0) and with sea salts concentrations of 1-10 % (w/v) (optimum 3 % sea salts) and NaCl concentrations of 1.5-5.0 % (w/v) (optimum 2.5 % NaCl). Under optimal growth conditions, the generation time was around 55 min. The isolate was an obligate chemoorganoheterotroph, utilizing complex organic compounds, amino acids, carbohydrates and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamate, methionine, threonine, fructose, mannose, galactose, glucose, palatinose, rhamnose, turanose, gentiobiose, xylose, sorbose, pyruvate, tartaric acid, α-ketobutyric acid, α-ketovaleric acid, galacturonic acid and glucosaminic acid. Strain DY22619T was strictly anaerobic and facultatively dependent on various forms of Fe(III) as an electron acceptor: insoluble forms and soluble forms. It did not reduce sulfite, sulfate, thiosulfate or nitrate. The genomic DNA G+C content was 29.0 mol%. Phylogenetic 16S rRNA gene sequence analyses revealed that the closest relative of strain DY22619T was Caloranaerobacter azorensis MV1087T, sharing 97.41 % 16S rRNA gene sequence similarity. On the basis of physiological distinctness and phylogenetic distance, the isolate is considered to represent a novel species of the genus Caloranaerobacter, for which the name Caloranaerobacterhttp://dx.doi.org/10.1601/nm.4081ferrireducens sp. nov. is proposed. The type strain is DY22619T ( = JCM 19467T = DSM 27799T = MCCC1A06455T).
巻・号 65(Pt 6)
ページ 1714-1718
公開日 2015-6-1
DOI 10.1099/ijs.0.000165
PMID 25736413
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Ferric Compounds / metabolism Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification* Gram-Negative Anaerobic Straight, Curved, and Helical Rods / genetics Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification Hydrothermal Vents / microbiology* Iron Molecular Sequence Data Pacific Ocean Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sulfides
IF 2.415
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物  JCM 19467