| RRC ID |
40913
|
| 著者 |
Corral P, de la Haba RR, Sánchez-Porro C, Amoozegar MA, Papke RT, Ventosa A.
|
| タイトル |
Halorubrum persicum sp. nov., an extremely halophilic archaeon isolated from sediment of a hypersaline lake.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
An extremely halophilic archaeon belonging to the genus Halorubrum, strain C49T, was isolated from sediment of the hypersaline lake Aran-Bidgol in Iran. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain C49T was closely related to Halorubrum saccharovorum JCM 8865T (99.5 %) and other species of the genus Halorubrum. Studies based on multilocus sequence analysis revealed that strain C49T is placed among the species of Halorubrum; the strain constituted a defined branch in comparison with the type strains of species of Halorubrum, while the 16S rRNA gene sequence divergence could not define the status of the newly isolated strain. For optimum growth, strain C49T required 20 % (w/v) salts at pH 7.0 and 37 °C under aerobic conditions. Mg2+ was not required. The cells were pleomorphic rods, motile and stained Gram-variable. Colonies of the strain were pink. Hypotonic treatment with <12 % NaCl provoked cell lysis. The polar lipid pattern of strain C49T consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester derived from both C20C20 and C20C25 archaeol, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The DNA G+C content was 64.2 mol%. DNA-DNA hybridization studies and average nucleotide identity confirmed that strain C49T constitutes a distinct genospecies. Data obtained in this study show that strain C49T represents a novel species, for which the name Halorubrum persicum sp. nov. is proposed. The type strain is C49T ( = IBRC-M 10232T = JCM 30541T).
|
| 巻・号 |
65(Pt 6)
|
| ページ |
1770-1778
|
| 公開日 |
2015-6-1
|
| DOI |
10.1099/ijs.0.000175
|
| PMID |
25744586
|
| MeSH |
Base Composition
DNA, Archaeal / genetics
Fatty Acids / chemistry
Genes, Archaeal
Geologic Sediments / microbiology*
Halorubrum / classification*
Halorubrum / genetics
Halorubrum / isolation & purification
Iran
Lakes / microbiology*
Molecular Sequence Data
Multilocus Sequence Typing
Nucleic Acid Hybridization
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Salinity
Sequence Analysis, DNA
|
| IF |
2.415
|
| 引用数 |
10
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 8865
JCM 8891
JCM 13559
JCM 13560
JCM 13916
JCM 30541 |
