RRC ID 40938
著者 McGinnis JM, Cole JA, Dickinson MC, Mingle LA, Lapierre P, Musser KA, Wolfgang WJ.
タイトル Paracoccus sanguinis sp. nov., isolated from clinical specimens of New York State patients.
ジャーナル Int J Syst Evol Microbiol
Abstract Eight independent isolates of a Gram-reaction-negative, non-motile rod, were recovered from clinical specimens of New York State patients between the years 2005 and 2013. Four of these isolates were characterized in a taxonomic study using a polyphasic approach that involved phenotypic, phylogenetic and genotypic methodologies. Based on 16S rRNA gene sequence similarity and phylogenetic analysis, the closest relative type strain of the isolates is Paracoccus sphaerophysae HAMBI 3106T (97.7  % 16S rRNA gene sequence similarity). Among the four isolates, the 16S rRNA gene sequence similarity is 100 %. In silico genomic comparisons, including average nucleotide identity (ANI) and the genome-to-genome distance calculator (GGDC), were used as an alternative to DNA-DNA hybridization in this study to support designation of the four isolates as a novel species of the genus Paracoccus. Mass spectrometry profiles were also obtained for the novel isolates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The predominant cellular fatty acids of the novel isolates were C18 : 1ω7c and C18 : 0. Biochemical analysis and morphological characteristics further contribute to designation of the four isolates as a novel species of the genus Paracoccus, for which the name Paracoccus sanguinis sp. nov. is proposed. The type strain is 05503T( = DSM 29303T = LMG 28451T).
巻・号 65(Pt 6)
ページ 1877-1882
公開日 2015-6-1
DOI 10.1099/ijs.0.000193
PMID 25792366
MeSH Aged Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Female Humans Male Middle Aged Molecular Sequence Data New York Nucleic Acid Hybridization Paracoccus / classification* Paracoccus / genetics Paracoccus / isolation & purification Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 2.415
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 14014