RRC ID 41049
著者 Wang B, Ji SQ, Tian XX, Qu LY, Li FL.
タイトル Brassicibacter thermophilus sp. nov., a thermophilic bacterium isolated from coastal sediment.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel thermophilic, obligately anaerobic bacterium, strain Cel2f(T), was isolated from a cellulolytic community enriched from coastal marine sediment. Cells were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Optimal growth temperature and pH of strain Cel2f(T) were 55 °C and pH 7.0, respectively. NaCl was essential for the growth of strain Cel2f(T) and the strain showed enhanced growth in the presence of sea salt; the optimum sea salt concentration for growth was 7% (w/v). Thiosulfate, sulfate and sulfite were potential electron acceptors. The major fatty acids of strain Cel2f(T) were iso-C15 : 0, C16 : 0, and C18 : 0. Polar lipid analysis indicated the presence of phosphatidylethanolamine and phosphatidylglycerol. Strain Cel2f(T) contained menaquinone MK-7 as the isoprenoid quinone, and the DNA G+C content was 31.3 mol%. Phylogenetic analysis revealed that the nearest relative of strain Cel2f(T) was Brassicibacter mesophilus BM(T) with 93.8% 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain Cel2f(T) represents a novel species of genus Brassicibacter, for which the name Brassicibacter thermophilus sp. nov. is proposed. The type strain is Cel2f(T) ( = JCM 30480(T) = CGMCC 1.5200(T)).
巻・号 65(9)
ページ 2870-2874
公開日 2015-9-1
DOI 10.1099/ijs.0.000348
PMID 25999591
MeSH Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Firmicutes / classification* Firmicutes / genetics Firmicutes / isolation & purification Geologic Sediments / microbiology* Molecular Sequence Data Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Seawater Sequence Analysis, DNA Sulfates / chemistry Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 5
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30480