論文 - 詳細
| RRC ID | 41053 |
|---|---|
| 著者 | Maeda AH, Kunihiro M, Ozeki Y, Nogi Y, Kanaly RA. |
| タイトル | Sphingobium barthaii sp. nov., a high molecular weight polycyclic aromatic hydrocarbon-degrading bacterium isolated from cattle pasture soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-stain-negative, yellow, rod-shaped bacterium, designated strain KK22(T), was isolated from a microbial consortium that grew on diesel fuel originally recovered from cattle pasture soil. Strain KK22(T) has been studied for its ability to biotransform high molecular weight polycyclic aromatic hydrocarbons. On the basis of 16S rRNA gene sequence phylogeny, strain KK22(T) was affiliated with the genus Sphingobium in the phylum Proteobacteria and was most closely related to Sphingobium fuliginis TKP(T) (99.8%) and less closely related to Sphingobium quisquiliarum P25(T) (97.5%). Results of DNA-DNA hybridization (DDH) revealed relatedness values between strain KK22(T) and strain TKP(T) and between strain KK22(T) and strain P25(T) of 21 ± 4% (reciprocal hybridization, 27 ± 2%) and 15 ± 2% (reciprocal hybridization, 17 ± 1%), respectively. Chemotaxonomic analyses of strain KK22(T) showed that the major respiratory quinone was ubiquinone Q-10, that the polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidyl-N-methylethylethanolamine and sphingoglycolipid, and that C18 : 1ω7c and C14 : 0 2-OH were the main fatty acid and hydroxylated fatty acids, respectively. This strain was unable to reduce nitrate and the genomic DNA G+C content was 64.7 mol%. Based upon the results of the DDH analyses, the fact that strain KK22(T) was motile, and its biochemical and physiological characteristics, strain KK22(T) could be separated from recognized species of the genus Sphingobium. We conclude that strain KK22(T) represents a novel species of this genus for which the name Sphingobium barthaii sp. nov. is proposed; the type strain is KK22(T) ( = DSM 29313(T) = JCM 30309(T)). |
| 巻・号 | 65(9) |
| ページ | 2919-2924 |
| 公開日 | 2015-9-1 |
| DOI | 10.1099/ijs.0.000356 |
| PMID | 26012583 |
| MeSH | Animals Bacterial Typing Techniques Base Composition Cattle DNA, Bacterial / genetics Fatty Acids / chemistry Molecular Sequence Data Molecular Weight Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny Pigmentation Polycyclic Aromatic Hydrocarbons / metabolism* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Soil Pollutants / metabolism* Sphingomonadaceae / classification* Sphingomonadaceae / genetics Sphingomonadaceae / isolation & purification Ubiquinone / chemistry |
| IF | 2.415 |
| 引用数 | 13 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 30309 |