RRC ID 4114
Author Higashijima S.
Title Transgenic zebrafish expressing fluorescent proteins in central nervous system neurons.
Journal Dev. Growth Differ.
Abstract Zebrafish is a powerful model system for investigations of vertebrate neural development. The animal has also become an important model for studies of neuronal function. Both in developmental and functional studies, transgenic zebrafish expressing fluorescent proteins in central nervous system neurons have been playing important roles. We review here the methods for producing transgenic zebrafish. Recent advances in transposon- or bacterial artificial chromosome-based transgenesis greatly facilitate the creation of useful lines. We also present our study on alx-positive neurons to reveal how transgenic zebrafish expressing fluorescent proteins in a specific class of neurons can be used to investigate their development and function.
Volume 50(6)
Pages 407-13
Published 2008-8
DOI 10.1111/j.1440-169X.2008.01023.x
PII DGD1023
PMID 18445066
MeSH Animals Animals, Genetically Modified* Central Nervous System / metabolism* Chromosomes, Artificial, Bacterial Fluorescent Dyes / pharmacology Genetic Techniques Green Fluorescent Proteins / metabolism Luminescent Proteins / metabolism Luminescent Proteins / pharmacology Models, Biological Models, Genetic Neurons / metabolism Transgenes Zebrafish / genetics*
IF 1.638
Times Cited 18
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
Zebrafish Review