RRC ID 41200
著者 Phongsopitanun W, Kudo T, Mori M, Shiomi K, Pittayakhajonwut P, Suwanborirux K, Tanasupawat S.
タイトル Micromonospora fluostatini sp. nov., isolated from marine sediment.
ジャーナル Int J Syst Evol Microbiol
Abstract The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003T represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H4). Cellular fatty acids comprised C18 : 1ω9c, iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0. On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10T (99.38 %), Micromonospora chaiyaphumensis MC5-1T (99.16 %), Micromonospora yangpuensis FXJ6.011T (98.97 %), Micromonospora echinaurantiaca DSM 43904T (98.97 %), Micromonospora pallida DSM 43817T (98.97 %), Micromonospora sagamiensis DSM 43912T and Micromonospora auratinigra JCM 12357T (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA-DNA relatedness values among strain PWB-003T and related type strains ranged from 11.3 ± 1.3 to 38.8 ± 1.1 %. On the basis of these observations, strain PWB-003T could be distinguished from its closely related type strains and is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB-003T = JCM 30529T = PCU 341T = TISTR 2345T) is proposed.
巻・号 65(12)
ページ 4417-4423
公開日 2015-12-1
DOI 10.1099/ijsem.0.000589
PMID 26358439
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Geologic Sediments / microbiology* Micromonospora / classification* Micromonospora / genetics Micromonospora / isolation & purification Molecular Sequence Data Peptidoglycan / chemistry Phospholipids / chemistry Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Thailand Vitamin K 2 / chemistry
IF 2.415
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 3133 JCM 3257 JCM 3310 JCM 18319 JCM 30529