| RRC ID |
41223
|
| Author |
Hyun DW, Oh SJ, Kim MS, Whon TW, Jung MJ, Shin NR, Kim PS, Kim HS, Lee JY, Kang W, Bae JW.
|
| Title |
Simplicispira piscis sp. nov., isolated from the gut of a Korean rockfish, Sebastes schlegelii.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A novel Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated strain RSG39T, was isolated from the gut of a Korean rockfish, Sebastes schlegelii. The 16S rRNA gene sequence analysis revealed that strain RSG39T belonged to the genus Simplicispira in the class Betaproteobacteria and its highest sequence similarity was shared with S. psychrophila (98.4 %). The isolate grew optimally at 20 °C, at pH 7 and with 0 % (w/v) NaCl. The main respiratory quinone of the isolate was ubiquinone Q-8. The major cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The polar lipids of the isolate were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and six unidentified lipids. The DNA-DNA hybridization values showed < 7.4 % genomic relatedness with closely related strains. The genomic DNA G+C content was 65.2 mol %. Based on phylogenetic, phenotypic, chemotaxonomic and genotypic analyses, strain RSG39T represents a novel species of the genus Simplicispira, for which the name Simplocospira piscis sp. nov. is proposed. The type strain is RSG39T ( = KACC 17539T = JCM 19291T).
|
| Volume |
65(12)
|
| Pages |
4689-4694
|
| Published |
2015-12-1
|
| DOI |
10.1099/ijsem.0.000635
|
| PMID |
26400548
|
| MeSH |
Animals
Bacterial Typing Techniques
Base Composition
Comamonadaceae / classification*
Comamonadaceae / genetics
Comamonadaceae / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / chemistry
Intestines / microbiology*
Molecular Sequence Data
Nucleic Acid Hybridization
Perciformes / microbiology*
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Republic of Korea
Sequence Analysis, DNA
Ubiquinone / chemistry
|
| IF |
2.415
|
| Times Cited |
7
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 19291 |
