RRC ID |
41237
|
Author |
Dekio I, Culak R, Misra R, Gaulton T, Fang M, Sakamoto M, Ohkuma M, Oshima K, Hattori M, Klenk HP, Rajendram D, Gharbia SE, Shah HN.
|
Title |
Dissecting the taxonomic heterogeneity within Propionibacterium acnes: proposal for Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov.
|
Journal |
Int J Syst Evol Microbiol
|
Abstract |
Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, l-pyrrolidonyl arylamidase or d-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T ( = ATCC 6919T = JCM 6425T = DSM 1897T = CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T ( = NCTC 13655T = JCM 18919T).
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Volume |
65(12)
|
Pages |
4776-4787
|
Published |
2015-12-1
|
DOI |
10.1099/ijsem.0.000648
|
PMID |
26432704
|
MeSH |
Bacterial Proteins / chemistry
Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / chemistry
Genes, Bacterial
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
Propionibacterium acnes / classification*
RNA, Ribosomal, 16S / genetics
Ribosomal Proteins / chemistry
Sequence Analysis, DNA
Skin / microbiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
IF |
2.415
|
Times Cited |
29
|
WOS Category
|
MICROBIOLOGY
|
Resource |
General Microbes |
JCM 6495
JCM 6473
JCM 18907
JCM 18909
JCM 18916
JCM 18918
JCM 18919
JCM 18920
JCM 18925
JCM 18926
JCM 21245 |