RRC ID 41312
Author Kanatsu-Shinohara M, Naoki H, Shinohara T.
Title Nonrandom Germline Transmission of Mouse Spermatogonial Stem Cells.
Journal Dev. Cell
Abstract Genes are thought to be transmitted to offspring by random fertilization of a small number of oocytes with numerous spermatozoa. Here we analyzed the dynamics of male germline transmission by genetic marking and transplantation of spermatogonial stem cells (SSCs). We found that offspring deriving from a small number of specific SSCs appear within a limited time. Interestingly, the same SSC clones reappear later with an average functional lifespan of ∼124.4 days. Cyclic offspring production from SSCs was not caused by changes in SSC self-renewal activity because lineage-tracing analyses suggested that all SSCs actively proliferated. Selection appears to occur during the differentiating spermatogonia stage, when extensive apoptosis was observed. The pattern of germline transmission could be predicted using a mathematical model in which SSCs repeat cycles of transient spermatogenic burst and refractory periods. Thus, spermatogenesis is a regulated process whereby specific SSC clones are repeatedly recruited for fertilization with long-term cycles.
Volume 38(3)
Pages 248-61
Published 2016-8-8
DOI 10.1016/j.devcel.2016.07.011
PII S1534-5807(16)30471-3
PMID 27505415
MeSH Animals Apoptosis Cell Differentiation* Cell Proliferation Cells, Cultured Infertility / physiopathology* Male Mice Mice, Inbred C57BL Mice, Transgenic Models, Theoretical Spermatogenesis / physiology* Spermatogonia / cytology Spermatogonia / physiology* Stem Cell Transplantation* Stem Cells / cytology Stem Cells / physiology*
IF 9.616
Times Cited 1
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
DNA material CSII-EF-MCS-IRES2-Venus (RDB04384)