RRC ID 41315
Author Sakai H, Sumitani M, Chikami Y, Yahata K, Uchino K, Kiuchi T, Katsuma S, Aoki F, Sezutsu H, Suzuki MG.
Title Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.
Journal PLoS Genet.
Abstract In Bombyx mori (B. mori), Fem piRNA originates from the W chromosome and is responsible for femaleness. The Fem piRNA-PIWI complex targets and cleaves mRNAs transcribed from the Masc gene. Masc encodes a novel CCCH type zinc-finger protein and is required for male-specific splicing of B. mori doublesex (Bmdsx) transcripts. In the present study, several silkworm strains carrying a transgene, which encodes a Fem piRNA-resistant Masc mRNA (Masc-R), were generated. Forced expression of the Masc-R transgene caused female-specific lethality during the larval stages. One of the Masc-R strains weakly expressed Masc-R in various tissues. Females heterozygous for the transgene expressed male-specific isoform of the Bombyx homolog of insulin-like growth factor II mRNA-binding protein (ImpM) and Bmdsx. All examined females showed a lower inducibility of vitellogenin synthesis and exhibited abnormalities in the ovaries. Testis-like tissues were observed in abnormal ovaries and, notably, the tissues contained considerable numbers of sperm bundles. Homozygous expression of the transgene resulted in formation of the male-specific abdominal segment in adult females and caused partial male differentiation in female genitalia. These results strongly suggest that Masc is an important regulatory gene of maleness in B. mori.
Volume 12(8)
Pages e1006203
Published 2016-8
DOI 10.1371/journal.pgen.1006203
PII PGENETICS-D-16-00853
PMID 27579676
PMC PMC5007099
MeSH Animals Animals, Genetically Modified Bombyx / genetics* Bombyx / growth & development DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics* Female Gene Expression Regulation, Developmental Insect Proteins / biosynthesis Insect Proteins / genetics* Insulin-Like Growth Factor II / biosynthesis Insulin-Like Growth Factor II / genetics* Male Ovary / growth & development Ovary / metabolism RNA Splicing / genetics RNA, Small Interfering / biosynthesis RNA, Small Interfering / genetics* Sex Chromosomes / genetics Sex Determination Processes / genetics* Testis / growth & development Testis / metabolism Vitellogenins / biosynthesis Vitellogenins / genetics
IF 5.54
Resource
Silkworms Suzu19-1