論文 - 詳細
| RRC ID | 41338 |
|---|---|
| 著者 | Watai Y, Sase I, Shiono H, Nakano Y. |
| タイトル | Regulation of nuclear import by light-induced activation of caged nuclear localization signal in living cells. |
| ジャーナル | FEBS Lett |
| Abstract |
A novel fluorescence probe suitable for the study of nuclear import in living cells has been developed. The lysine-128 residue in SV40 T-antigen nuclear localization signal (NLS) was converted to a caged lysine with the amino acid blocked by a photocleavable protecting group. Following irradiation of ultraviolet (UV) light, the caged NLS conjugate translocated into and accumulated in the nucleus within 20 min similar to uncaged NLS conjugate. Maximum import rate saturated approximately 4.78+/-0.21% per minute when the duration of irradiation was more than 1/15 s (22 mW/cm(2)). Caged NLS conjugate tended to distribute near the surface of the nucleus, and this association became stronger after UV irradiation. The caged conjugate enabled us to regulate the initial state of the reaction, both spatially and temporally. |
| 巻・号 | 488(1-2) |
| ページ | 39-44 |
| 公開日 | 2001-1-12 |
| DOI | 10.1016/s0014-5793(00)02399-1 |
| PII | S0014-5793(00)02399-1 |
| PMID | 11163792 |
| MeSH | Active Transport, Cell Nucleus / radiation effects Amino Acid Sequence Animals Antigens, Viral, Tumor / chemistry Antigens, Viral, Tumor / genetics Cattle Cell Survival Fluorescent Dyes / chemical synthesis Fluorescent Dyes / chemistry Fluorescent Dyes / metabolism* Fluorescent Dyes / radiation effects* HeLa Cells Humans Kinetics Light* Lysine / chemistry Lysine / metabolism Microscopy, Fluorescence Nuclear Localization Signals / chemical synthesis Nuclear Localization Signals / chemistry Nuclear Localization Signals / metabolism* Nuclear Localization Signals / radiation effects* Oligopeptides / chemical synthesis Oligopeptides / chemistry Oligopeptides / metabolism* Oligopeptides / radiation effects* Photolysis / radiation effects Recombinant Fusion Proteins / metabolism Serum Albumin / metabolism Simian virus 40 |
| IF | 3.057 |
| 引用数 | 16 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |