| RRC ID |
41338
|
| 著者 |
Watai Y, Sase I, Shiono H, Nakano Y.
|
| タイトル |
Regulation of nuclear import by light-induced activation of caged nuclear localization signal in living cells.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
A novel fluorescence probe suitable for the study of nuclear import in living cells has been developed. The lysine-128 residue in SV40 T-antigen nuclear localization signal (NLS) was converted to a caged lysine with the amino acid blocked by a photocleavable protecting group. Following irradiation of ultraviolet (UV) light, the caged NLS conjugate translocated into and accumulated in the nucleus within 20 min similar to uncaged NLS conjugate. Maximum import rate saturated approximately 4.78+/-0.21% per minute when the duration of irradiation was more than 1/15 s (22 mW/cm(2)). Caged NLS conjugate tended to distribute near the surface of the nucleus, and this association became stronger after UV irradiation. The caged conjugate enabled us to regulate the initial state of the reaction, both spatially and temporally.
|
| 巻・号 |
488(1-2)
|
| ページ |
39-44
|
| 公開日 |
2001-1-12
|
| DOI |
10.1016/s0014-5793(00)02399-1
|
| PII |
S0014-5793(00)02399-1
|
| PMID |
11163792
|
| MeSH |
Active Transport, Cell Nucleus / radiation effects
Amino Acid Sequence
Animals
Antigens, Viral, Tumor / chemistry
Antigens, Viral, Tumor / genetics
Cattle
Cell Survival
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry
Fluorescent Dyes / metabolism*
Fluorescent Dyes / radiation effects*
HeLa Cells
Humans
Kinetics
Light*
Lysine / chemistry
Lysine / metabolism
Microscopy, Fluorescence
Nuclear Localization Signals / chemical synthesis
Nuclear Localization Signals / chemistry
Nuclear Localization Signals / metabolism*
Nuclear Localization Signals / radiation effects*
Oligopeptides / chemical synthesis
Oligopeptides / chemistry
Oligopeptides / metabolism*
Oligopeptides / radiation effects*
Photolysis / radiation effects
Recombinant Fusion Proteins / metabolism
Serum Albumin / metabolism
Simian virus 40
|
| IF |
3.057
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
