論文 - 詳細
| RRC ID | 41355 |
|---|---|
| 著者 | Kawasaki A, Matsumura I, Miyagawa Ji, Ezoe S, Tanaka H, Terada Y, Tatsuka M, Machii T, Miyazaki H, Furukawa Y, Kanakura Y. |
| タイトル | Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells. |
| ジャーナル | J Cell Biol |
| Abstract |
During the late phase of megakaryopoiesis, megakaryocytes undergo polyploidization, which is characterized by DNA duplication without concomitant cell division. However, it remains unknown by which mechanisms this process occurs. AIM-1 and STK15 belong to the Aurora/increase-in-ploidy (Ipl)1 serine/threonine kinase family and play key roles in mitosis. In a human interleukin-3-dependent cell line, F-36P, the expressions of AIM-1 and STK15 mRNA were specifically observed at G2/M phase of the cell cycle during proliferation. In contrast, the expressions of AIM-1 and STK15 were continuously repressed during megakaryocytic polyploidization of human erythro/megakaryocytic cell lines (F-36P, K562, and CMK) treated with thrombopoietin, activated ras (H-ras(G12V)), or phorbol ester. Furthermore, their expressions were suppressed during thrombopoietin-induced polyploidization of normal human megakaryocytes. Activation of AIM-1 by the induced expression of AIM-1(wild-type) canceled TPA-induced polyploidization of K562 cells significantly, whereas that of STK15 did not. Moreover, suppression of AIM-1 by the induced expression of AIM-1 (K/R, dominant-negative type) led to polyploidization in 25% of K562 cells, whereas STK15(K/R) showed no effect. Also, the induced expression of AIM-1(K/R) in CMK cells provoked polyploidization up to 32N. These results suggested that downregulation of AIM-1 at M phase may be involved in abortive mitosis and polyploid formation of megakaryocytes. |
| 巻・号 | 152(2) |
| ページ | 275-87 |
| 公開日 | 2001-1-22 |
| DOI | 10.1083/jcb.152.2.275 |
| PMID | 11266445 |
| PMC | PMC2199624 |
| MeSH | Animals Aurora Kinase A Aurora Kinases Bone Marrow Cells / cytology Cell Cycle / physiology* Cell Division Cell Line Cells, Cultured DNA Replication Erythropoiesis / physiology* Female Gene Expression Regulation, Enzymologic* Genes, ras Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / drug effects Hematopoietic Stem Cells / physiology* Humans Interleukin-3 / pharmacology Megakaryocytes / cytology Megakaryocytes / drug effects Megakaryocytes / physiology* Mice Mice, Inbred C57BL Phorbol Esters / pharmacology Polyploidy* Protein Kinases / genetics* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism RNA, Messenger / genetics Recombinant Proteins / pharmacology Stem Cell Factor / pharmacology Thrombopoietin / pharmacology Transcription, Genetic |
| IF | 8.811 |
| 引用数 | 51 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) F-36P(RCB0775) |