Reference - Detail
| RRC ID | 41386 |
|---|---|
| Author | Sugimoto C, Fujieda S, Sunaga H, Noda I, Tanaka N, Kimura Y, Saito H, Matsukawa S. |
| Title | Granulocyte colony-stimulating factor (G-CSF)-mediated signaling regulates type IV collagenase activity in head and neck cancer cells. |
| Journal | Int J Cancer |
| Abstract |
Granulocyte colony-stimulating factor (G-CSF), a hematopoietic cytokine, regulates the proliferation and differentiation of granulocytic progenitor cells and functionally activated mature neutrophils. G-CSF also affects nonhematopoietic tumor cells through its binding to the specific receptor (G-CSFR) on the cells. The type IV collagenase [matrix metalloproteinase 2 (MMP-2)] is known to play a main role in the process of invasion and metastasis, but its regulation, for example, in expression or in activation, is not clearly understood. In this study, we investigated the role of G-CSF in the regulation of tumor cell invasion and the synthesis of MMP-2. G-CSFs producing the head and neck carcinoma cell line T3M-1 cells with metastatic ability and no G-CSF receptor (G-CSFR) expression were transfected with G-CSFR expression vector. In vitro treatment of G-CSFR-transfectant T3M-1 cells with recombinant G-CSF (rG-CSF) significantly augmented their invasive potential in a reconstituted basement membrane (Matrigel) system compared with that of parental cells. Moreover, MMP-2 activity of G-CSFR-transfectant T3M-1 cells was enhanced by the stimulation with rG-CSF, as assessed by gelatin zymography. These results identify G-CSF as a regulator of MMP-2 and cellular invasion. |
| Volume | 93(1) |
| Pages | 42-6 |
| Published | 2001-7-1 |
| DOI | 10.1002/ijc.1297 |
| PII | 10.1002/ijc.1297 |
| PMID | 11391619 |
| MeSH | Carcinoma, Squamous Cell / pathology Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Neoplastic / drug effects Granulocyte Colony-Stimulating Factor / pharmacology* Head and Neck Neoplasms Humans Matrix Metalloproteinase 2 / genetics* Mouth Neoplasms / pathology Neoplasm Invasiveness RNA, Messenger / genetics Receptors, Granulocyte Colony-Stimulating Factor / drug effects Receptors, Granulocyte Colony-Stimulating Factor / genetics Receptors, Granulocyte Colony-Stimulating Factor / physiology* Recombinant Proteins / drug effects Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Signal Transduction / physiology* Transcription, Genetic / drug effects Transfection Tumor Cells, Cultured / pathology |
| IF | 5.145 |
| Times Cited | 18 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | T3M-1 |