RRC ID 41391
Author Kuwahara M, Takeda M, Takeuchi Y, Kuwahara M, Harada T, Maita K.
Title Transforming growth factor beta production by spontaneous malignant mesothelioma cell lines derived from Fisher 344 rats.
Journal Virchows Arch
Abstract We investigated whether transforming growth factor beta (TGF-beta) is involved in the growth of malignant mesothelioma (MM) cells in culture. TGF-beta production was examined in two mesothelioma cell lines (MeET-4 and -6) that were established from rat spontaneous MM in our laboratory. TGF-beta bioactivity in conditioned medium of these cell lines was analyzed using a CCL64 mink lung epithelial cell growth inhibition assay and found to be 30-70 times higher than that of normal rat mesothelial cells (MCs). The MM cell lines also showed considerably higher levels of TGF-beta mRNA expression when compared with MCs. The bioactivity and mRNA expression level were greater in MeET-4 than MeET-6. When MeET-4 was treated with antisense TGF-beta1 oligonucleotide (ODN), a significant decrease in both anchorage-dependent and -independent growth was observed. Treatment with exogenous TGF-beta resulted in no effects on the growth pattern of the MM cell lines, while proliferation of the MCs was slightly induced. It is considered that TGF-beta appears to be produced by rat spontaneous MM cells through an autocrine mechanism and could modulate the malignant growth of the tumor cells.
Volume 438(5)
Pages 492-7
Published 2001-5-1
DOI 10.1007/s004280000363
PMID 11407478
MeSH Animals Cell Count Cell Division / drug effects Culture Media, Conditioned / pharmacology DNA Primers / chemistry Dose-Response Relationship, Drug Epithelium / drug effects Mesothelioma / genetics Mesothelioma / metabolism* Mesothelioma / pathology Molecular Sequence Data Oligonucleotides, Antisense / pharmacology Peritoneum / cytology Polymerase Chain Reaction RNA, Messenger / biosynthesis RNA, Neoplasm / biosynthesis Rats Rats, Inbred F344 Retroperitoneal Neoplasms / genetics Retroperitoneal Neoplasms / metabolism* Retroperitoneal Neoplasms / pathology Transforming Growth Factor beta / biosynthesis* Transforming Growth Factor beta / genetics Tumor Cells, Cultured / metabolism* Tumor Cells, Cultured / pathology
IF 2.906
Times Cited 11
WOS Category PATHOLOGY
Resource
Human and Animal Cells Mv.1.Lu(NBL-7)(RCB0996)