RRC ID 414
Author Kimura Y, Corcoran EE, Eto K, Gengyo-Ando K, Muramatsu MA, Kobayashi R, Freedman JH, Mitani S, Hagiwara M, Means AR, Tokumitsu H.
Title A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans.
Journal EMBO Rep.
Abstract Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes.
Volume 3(10)
Pages 962-6
Published 2002-10
DOI 10.1093/embo-reports/kvf191
PII kvf191
PMID 12231504
PMC PMC1307624
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Blotting, Western Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Calcium / metabolism Calcium-Calmodulin-Dependent Protein Kinases / metabolism* Cyclic AMP Response Element-Binding Protein / metabolism* DNA, Complementary / metabolism Electrophoresis, Polyacrylamide Gel Exons Gene Library Humans Mice Molecular Sequence Data Mutagenesis Neurons / metabolism Open Reading Frames Phosphorylation Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Transcription, Genetic
IF 8.383
Times Cited 63
C.elegans tm421