論文 - 詳細
| RRC ID | 41430 |
|---|---|
| 著者 | Nagase M, Alam MM, Tsushima A, Yoshizawa T, Sakato N. |
| タイトル | Apoptosis induction by T-2 toxin: activation of caspase-9, caspase-3, and DFF-40/CAD through cytosolic release of cytochrome c in HL-60 cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
The molecules participating in apoptosis induced by T-2 toxin in human leukemia HL-60 cells were investigated. The rank order of the potency of trichothecene mycotoxins to induce internucleosomal DNA fragmentation was found to be T-2, satratoxin G, roridin A > diacetoxyscirpenol > baccharin B-5 > nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenon-X, baccharin B-4=vehicle control. Western blot analysis of caspase-3 in T-2-treated cells clearly indicated the appearance of its catalytically active fragment of 17-kDa. Increased caspase-3 activity was also detected by using a fluorogenic substrate, DEVD-AMC. Next, cells exposed to T-2 led to cleavage of PARP from its native 116-kDa form to the 85-kDa product. Moreover, DFF-45/ICAD were cleaved to give a 12.5-kDa fragment via T-2 treatment. T-2 caused the release of cytochrome c from mitochondria into the cytosol. Increased enzymic activity of caspase-9 on LEHD-AMC was shown. These data indicate that T-2-induced apoptosis involves activation of caspase-3 and DFF-40/CAD through cytosolic accumulation of cytochrome c along with caspase-9 activation. |
| 巻・号 | 65(8) |
| ページ | 1741-7 |
| 公開日 | 2001-8-1 |
| DOI | 10.1271/bbb.65.1741 |
| PMID | 11577712 |
| MeSH | Apoptosis / drug effects* Blotting, Western Caspase 3 Caspase 9 Caspases / metabolism* Cytochrome c Group / metabolism* Cytosol / drug effects Cytosol / enzymology* DNA Fragmentation Deoxyribonucleases / metabolism* Electrophoresis, Polyacrylamide Gel Enzyme Activation / drug effects HL-60 Cells Humans Indicators and Reagents Mitochondria / drug effects Mitochondria / enzymology Nucleosomes / chemistry Nucleosomes / drug effects Poly-ADP-Ribose Binding Proteins T-2 Toxin / toxicity* |
| IF | 1.516 |
| 引用数 | 48 |
| WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |