| RRC ID |
41444
|
| 著者 |
Dekigai H, Nakamura H, Bai J, Tanito M, Masutani H, Hirota K, Matsui H, Murakami M, Yodoi J.
|
| タイトル |
Geranylgeranylacetone promotes induction and secretion of thioredoxin in gastric mucosal cells and peripheral blood lymphocytes.
|
| ジャーナル |
Free Radic Res
|
| Abstract |
Thioredoxin (TRX) is a redox-active protein which is induced by oxidative stresses and shows a variety of biological activities including cytoprotection against oxidative stress. We recently reported that geranylgeranylacetone (GGA), an anti-ulcer drug, induces TRX in rat hepatocytes. In this study, we demonstrate that GGA promotes induction and secretion of TRX in rat gastric mucosal cells and human peripheral blood lymphocytes (PBLs). Western blotting and a sensitive sandwich ELISA showed that TRX was induced by GGA in the cell lysates and culture supernatants of rat gastric mucosal RGM-1 cells and human PBLs. LDH releasing assay showed that GGA protected rat gastric mucosal RGM-1 cells from ethanol-induced cytotoxicity. Moreover, exogenous recombinant wild type TRX decreased 51Cr release from primary cultured rat gastric mucosal cells incubated with ethanol or hydrogen peroxide in a dose-dependent manner, whereas recombinant mutant TRX (C32S/C35S), in which the two cysteines were replaced with serines in its active site, did not. These results indicate that GGA promotes the induction and secretion of TRX in a variety of types of cells and suggest that induced or secreted TRX may play an important role in the cytoprotective action of GGA on gastric mucosal cells.
|
| 巻・号 |
35(1)
|
| ページ |
23-30
|
| 公開日 |
2001-7-1
|
| DOI |
10.1080/10715760100300561
|
| PMID |
11697114
|
| MeSH |
Animals
Anti-Ulcer Agents / pharmacology*
Blotting, Western
Cell Line
Cytoprotection
Diterpenes / pharmacology*
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa / drug effects*
Gastric Mucosa / metabolism
Humans
L-Lactate Dehydrogenase / metabolism
Lymphocytes / drug effects*
Lymphocytes / metabolism
Rats
Thioredoxins / metabolism*
|
| IF |
2.839
|
| 引用数 |
30
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
