Reference - Detail
| RRC ID | 41448 |
|---|---|
| Author | Kamiya H, Murata-Kamiya N, Karino N, Ueno Y, Matsuda A, Kasai H. |
| Title | Induction of T --> G and T --> A transversions by 5-formyluracil in mammalian cells. |
| Journal | Mutat Res |
| Abstract |
Oxidatively damaged thymine, 5-formyluracil (5-fU), was incorporated into a predetermined site of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-CFTAAG-3' and 5'-CTFAAG-3' (F represents 5-fU), the recognition site for the restriction enzyme AflII (5'-CTTAAG-3'). The 5-fU was incorporated into a template strand of either the leading or lagging strand of DNA replication. The modified DNAs were transfected into simian COS-7 cells, and the DNAs replicated in the cells were recovered and were analyzed after the second transfection into Escherichia coli. The 5-fU did not block DNA replication in mammalian cells. The 5-fU residues were weakly mutagenic, and their mutation frequencies in double-stranded vectors were 0.01-0.04%. The T --> G and T --> A transversions were the mutations found most frequently, suggesting the formation of 5-fU.C and 5-fU.T base pairs, respectively. This is the first report that clearly shows the induction of transversion mutations by an oxidized pyrimidine base in DNA in mammalian cells. |
| Volume | 513(1-2) |
| Pages | 213-22 |
| Published | 2002-1-15 |
| DOI | 10.1016/s1383-5718(01)00312-6 |
| PII | S1383571801003126 |
| PMID | 11719107 |
| MeSH | Animals COS Cells Molecular Conformation Mutagens / toxicity* Mutation Uracil / analogs & derivatives* Uracil / chemistry Uracil / toxicity* |
| IF | 2.463 |
| Times Cited | 37 |
| WOS Category | TOXICOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |