| RRC ID |
41448
|
| Author |
Kamiya H, Murata-Kamiya N, Karino N, Ueno Y, Matsuda A, Kasai H.
|
| Title |
Induction of T --> G and T --> A transversions by 5-formyluracil in mammalian cells.
|
| Journal |
Mutat Res
|
| Abstract |
Oxidatively damaged thymine, 5-formyluracil (5-fU), was incorporated into a predetermined site of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-CFTAAG-3' and 5'-CTFAAG-3' (F represents 5-fU), the recognition site for the restriction enzyme AflII (5'-CTTAAG-3'). The 5-fU was incorporated into a template strand of either the leading or lagging strand of DNA replication. The modified DNAs were transfected into simian COS-7 cells, and the DNAs replicated in the cells were recovered and were analyzed after the second transfection into Escherichia coli. The 5-fU did not block DNA replication in mammalian cells. The 5-fU residues were weakly mutagenic, and their mutation frequencies in double-stranded vectors were 0.01-0.04%. The T --> G and T --> A transversions were the mutations found most frequently, suggesting the formation of 5-fU.C and 5-fU.T base pairs, respectively. This is the first report that clearly shows the induction of transversion mutations by an oxidized pyrimidine base in DNA in mammalian cells.
|
| Volume |
513(1-2)
|
| Pages |
213-22
|
| Published |
2002-1-15
|
| DOI |
10.1016/s1383-5718(01)00312-6
|
| PII |
S1383571801003126
|
| PMID |
11719107
|
| MeSH |
Animals
COS Cells
Molecular Conformation
Mutagens / toxicity*
Mutation
Uracil / analogs & derivatives*
Uracil / chemistry
Uracil / toxicity*
|
| IF |
2.463
|
| Times Cited |
37
|
|
WOS Category
|
TOXICOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
