Reference - Detail
RRC ID | 41454 |
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Author | Shimoaka T, Ogasawara T, Yonamine A, Chikazu D, Kawano H, Nakamura K, Itoh N, Kawaguchi H. |
Title | Regulation of osteoblast, chondrocyte, and osteoclast functions by fibroblast growth factor (FGF)-18 in comparison with FGF-2 and FGF-10. |
Journal | J Biol Chem |
Abstract |
This study investigated the actions of fibroblast growth factor (FGF)-18, a novel member of the FGF family, on osteoblasts, chondrocytes, and osteoclasts and compared them with those of FGF-2 and FGF-10. FGF-18 stimulated the proliferation of cultured mouse primary osteoblasts, osteoblastic MC3T3-E1 cells, primary chondrocytes, and prechondrocytic ATDC5 cells, although it inhibited the differentiation and matrix synthesis of these cells. FGF-18 up-regulated the phosphorylation of extracellular signal-regulated kinase in both osteoblasts and chondrocytes and up-regulated the phosphorylation of p38 mitogen-activated protein kinase only in chondrocytes. FGF-18 mitogenic actions were blocked by a specific inhibitor of extracellular signal-regulated kinase in both osteoblasts and chondrocytes and by a specific inhibitor of p38 mitogen-activated protein kinase in chondrocytes. With regard to the action of FGF-18 on bone resorption, FGF-18 not only induced osteoclast formation through receptor activator of nuclear factor-kappaB ligand and cyclooxygenase-2 but also stimulated osteoclast function to form resorbed pits on a dentine slice in the mouse coculture system. All these effects of FGF-18 bore a close resemblance to those of FGF-2, whereas FGF-10 affects none of these cells. FGF-18 may therefore compensate for the action of FGF-2 on bone and cartilage. |
Volume | 277(9) |
Pages | 7493-500 |
Published | 2002-3-1 |
DOI | 10.1074/jbc.M108653200 |
PII | S0021-9258(19)82310-4 |
PMID | 11741978 |
MeSH | Animals Blotting, Western Bone and Bones / metabolism Cartilage / metabolism Cell Differentiation Cell Division Cell Line Cell Nucleus / metabolism Cells, Cultured Chondrocytes / metabolism* Coculture Techniques Coloring Agents / pharmacology Cyclooxygenase 2 Dose-Response Relationship, Drug Fibroblast Growth Factor 10 Fibroblast Growth Factor 2 / metabolism* Fibroblast Growth Factors / metabolism* Fibroblast Growth Factors / pharmacology Isoenzymes / metabolism Ligands Mice Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism Osteoblasts / metabolism* Osteoclasts / metabolism* Phosphorylation Prostaglandin-Endoperoxide Synthases / metabolism Protein Binding Rats Signal Transduction Time Factors Tyrosine / metabolism Up-Regulation p38 Mitogen-Activated Protein Kinases |
IF | 4.238 |
Times Cited | 119 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells | ATDC5(RCB0565) |