RRC ID 41464
Author Buerstedde JM, Arakawa H, Watahiki A, Carninci PP, Hayashizaki YY, Korn B, Plachy J.
Title The DT40 web site: sampling and connecting the genes of a B cell line.
Journal Nucleic Acids Res
Abstract Thousands of new vertebrate genes have been discovered and genetic systems are needed to address their functions at the cellular level. The chicken B cell line DT40 allows efficient gene disruptions due to its high homologous recombination activity. However, cloning the gene of interest is often cumbersome, since relatively few chicken cDNA sequences are present in the public databases. In addition, the accumulation of multiple mutations within the same cell clone is limited by the consumption of one drug-resistance marker for each transfection. Here, we present the DT40 web site (http://genetics.hpi.uni-hamburg.de/dt40.html), which includes a comprehensive database of chicken bursal ESTs to identify disruption candidate genes and recyclable marker cassettes based on the loxP system. These freely available resources greatly facilitate the analysis of genes and genetic networks.
Volume 30(1)
Pages 230-1
Published 2002-1-1
DOI 10.1093/nar/30.1.230
PMID 11752301
PMC PMC99062
MeSH Animals B-Lymphocytes / immunology* Biomarkers / analysis Bursa of Fabricius / immunology* Cell Line Chickens / genetics Chickens / immunology* Databases, Genetic* Drug Resistance Expressed Sequence Tags Gene Library Gene Targeting Information Storage and Retrieval Internet Mutation
IF 11.502
Times Cited 26
Human and Animal Cells