| RRC ID |
41464
|
| 著者 |
Buerstedde JM, Arakawa H, Watahiki A, Carninci PP, Hayashizaki YY, Korn B, Plachy J.
|
| タイトル |
The DT40 web site: sampling and connecting the genes of a B cell line.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
Thousands of new vertebrate genes have been discovered and genetic systems are needed to address their functions at the cellular level. The chicken B cell line DT40 allows efficient gene disruptions due to its high homologous recombination activity. However, cloning the gene of interest is often cumbersome, since relatively few chicken cDNA sequences are present in the public databases. In addition, the accumulation of multiple mutations within the same cell clone is limited by the consumption of one drug-resistance marker for each transfection. Here, we present the DT40 web site (http://genetics.hpi.uni-hamburg.de/dt40.html), which includes a comprehensive database of chicken bursal ESTs to identify disruption candidate genes and recyclable marker cassettes based on the loxP system. These freely available resources greatly facilitate the analysis of genes and genetic networks.
|
| 巻・号 |
30(1)
|
| ページ |
230-1
|
| 公開日 |
2002-1-1
|
| DOI |
10.1093/nar/30.1.230
|
| PMID |
11752301
|
| PMC |
PMC99062
|
| MeSH |
Animals
B-Lymphocytes / immunology*
Biomarkers / analysis
Bursa of Fabricius / immunology*
Cell Line
Chickens / genetics
Chickens / immunology*
Databases, Genetic*
Drug Resistance
Expressed Sequence Tags
Gene Library
Gene Targeting
Information Storage and Retrieval
Internet
Mutation
|
| IF |
11.502
|
| 引用数 |
26
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
