Reference - Detail
|Author||Tang L, Ebara S, Kawasaki S, Wakabayashi S, Nikaido T, Takaoka K.|
|Title||FK506 enhanced osteoblastic differentiation in mesenchymal cells.|
|Journal||Cell Biol Int|
Bone morphogenetic protein (BMP) is a bone-derived growth factor capable of promoting the differentiation of mesenchymal cells into osteogenic lineage pathways. Recently, immunosuppressants were reported to cause a moderate increase in osteoblastic differentiation in a rat osteoblast-like osteosarcoma cell line. If immunosuppressants can induce osteoblastic differentiation, it will be useful for bone tissue transplantation. We assessed the effect of immunosuppressants with or without BMP-4 on inducing osteoblastic differentiation in osteoblast-like and other mesenchymal cells. FK506, an immunosuppressant often used clinically, induced a dose- and time-dependent increase in alkaline phosphatase (ALP) activity, one of the markers of osteoblast differentiation, in cells derived from mesenchyma. In the presence of BMP-4, ALP activity, mRNA levels of ALP and osteocalcin increased. FK506 was found to not only stimulate osteoblastic differentiation, but also to enhance BMP-4 induced osteoblastic differentiation. These results suggest that FK506 promotes differentiation of osteoblastic cells.
|MeSH||3T3 Cells Alkaline Phosphatase / metabolism Animals Blotting, Northern Bone Marrow Cells / metabolism Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / metabolism* Cell Differentiation Cell Division Cell Line Cells, Cultured Culture Media, Conditioned / pharmacology Cyclosporine / pharmacology DNA, Complementary / metabolism Dose-Response Relationship, Drug Immunosuppressive Agents / pharmacology* Mesoderm / cytology* Mice Mice, Inbred C3H Osteoblasts / metabolism* Osteocalcin / metabolism Osteosarcoma / metabolism Polymerase Chain Reaction RNA, Messenger / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Sirolimus / pharmacology Tacrolimus / pharmacology* Time Factors|
|WOS Category||CELL BIOLOGY|
|Human and Animal Cells||ST2(RCB0224) 10T1/2(RCB0247)|