RRC ID 41477
Author Tang L, Ebara S, Kawasaki S, Wakabayashi S, Nikaido T, Takaoka K.
Title FK506 enhanced osteoblastic differentiation in mesenchymal cells.
Journal Cell Biol Int
Abstract Bone morphogenetic protein (BMP) is a bone-derived growth factor capable of promoting the differentiation of mesenchymal cells into osteogenic lineage pathways. Recently, immunosuppressants were reported to cause a moderate increase in osteoblastic differentiation in a rat osteoblast-like osteosarcoma cell line. If immunosuppressants can induce osteoblastic differentiation, it will be useful for bone tissue transplantation. We assessed the effect of immunosuppressants with or without BMP-4 on inducing osteoblastic differentiation in osteoblast-like and other mesenchymal cells. FK506, an immunosuppressant often used clinically, induced a dose- and time-dependent increase in alkaline phosphatase (ALP) activity, one of the markers of osteoblast differentiation, in cells derived from mesenchyma. In the presence of BMP-4, ALP activity, mRNA levels of ALP and osteocalcin increased. FK506 was found to not only stimulate osteoblastic differentiation, but also to enhance BMP-4 induced osteoblastic differentiation. These results suggest that FK506 promotes differentiation of osteoblastic cells.
Volume 26(1)
Pages 75-84
Published 2002-1-1
DOI 10.1006/cbir.2001.0812
PII S1065699501908121
PMID 11779223
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Blotting, Northern Bone Marrow Cells / metabolism Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / metabolism* Cell Differentiation Cell Division Cell Line Cells, Cultured Culture Media, Conditioned / pharmacology Cyclosporine / pharmacology DNA, Complementary / metabolism Dose-Response Relationship, Drug Immunosuppressive Agents / pharmacology* Mesoderm / cytology* Mice Mice, Inbred C3H Osteoblasts / metabolism* Osteocalcin / metabolism Osteosarcoma / metabolism Polymerase Chain Reaction RNA, Messenger / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Sirolimus / pharmacology Tacrolimus / pharmacology* Time Factors
IF 2.571
Times Cited 52
Human and Animal Cells ST2(RCB0224) 10T1/2(RCB0247)