| RRC ID |
41498
|
| Author |
Yamashita Y, Shimada M, Tsujita E, Tanaka S, Ijima H, Nakazawa K, Sakiyama R, Fukuda J, Ueda T, Funatsu K, Sugimachi K.
|
| Title |
Polyurethane foam/spheroid culture system using human hepatoblastoma cell line (Hep G2) as a possible new hybrid artificial liver.
|
| Journal |
Cell Transplant
|
| Abstract |
The risk of xenozoonosis infections poses the greatest obstacle against the clinical application of hybrid artificial liver support system (HALSS). Primary human hepatocytes are an ideal source for HALSS, but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, we used human hepatocytes with replication capacity (fetal hepatocytes, Hep G2, and Huh 7) in a polyurethane foam (PUF)/spheroid culture system in vitro, and analyzed liver functions such as ammonia removal and albumin synthesis capacity; results were compared to those of porcine hepatocytes. Human fetal hepatocytes, Hep G2, and Huh 7 formed spheroids spontaneously within 24 h in a PUF/spheroid culture system; ammonia removal activity (micromol/10(6) nuclei/h) was upregulated, as was albumin synthesis activity (microg/10(6) nuclei/day). In particular, Hep G2 spheroids demonstrated high ammonia removal and albumin synthesis activities: 85% of the ammonia removal activity and 171.7% of the albumin synthesis activity of porcine hepatocytes in the monolayer culture. These results indicate the possibility of the development of a multicapillary PUF (MC-PUF) packed-bed culture system of hepatocyte spheroids as a HALSS using Hep G2.
|
| Volume |
10(8)
|
| Pages |
717-22
|
| Published |
2001-1-1
|
| PMID |
11814114
|
| MeSH |
Albumins / biosynthesis
Ammonia / metabolism
Animals
Cell Culture Techniques / methods*
Cell Division
Cells, Cultured
Culture Media
Hepatoblastoma / metabolism
Hepatoblastoma / pathology
Hepatocytes / cytology*
Hepatocytes / metabolism
Humans
Liver / embryology
Liver Neoplasms / metabolism
Liver Neoplasms / pathology
Liver, Artificial*
Polyurethanes / chemistry*
Spheroids, Cellular
Swine
Time Factors
Tumor Cells, Cultured
Up-Regulation
|
| IF |
3.341
|
| Times Cited |
33
|
|
WOS Category
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB0459)
HuH-7(RCB1366) |
