論文 - 詳細
| RRC ID | 41502 |
|---|---|
| 著者 | Okemoto K, Nakajima Y, Fujioka T, Natori S. |
| タイトル | Participation of two N-terminal residues in LPS-neutralizing activity of sarcotoxin IA. |
| ジャーナル | J Biochem |
| Abstract |
Sarcotoxin IA is a cecropin-type antibacterial peptide of flesh fly. Using a mutant sarcotoxin IA lacking two N-terminal residues, we demonstrated that these residues are indispensable for its antibacterial activity against Escherichia coli and LPS-binding. Contrary to the native sarcotoxin IA, the mutant sarcotoxin IA could not neutralize various biological activities of LPS. It was suggested that sarcotoxin IA firmly binds to the lipid A core of LPS via these two N-terminal residues and forms a stable binding complex that exhibits no appreciable biological activity like native LPS. |
| 巻・号 | 131(2) |
| ページ | 277-81 |
| 公開日 | 2002-2-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a003099 |
| PMID | 11820943 |
| MeSH | Amino Acids / metabolism Animals Anti-Infective Agents / chemical synthesis Anti-Infective Agents / metabolism* Anti-Infective Agents / pharmacology Blotting, Northern Cells, Cultured Diptera Glucose / metabolism Insect Proteins / genetics Insect Proteins / metabolism* Insect Proteins / pharmacology Lipopolysaccharides / metabolism* Liposomes / metabolism* Macrophages / metabolism Microbial Sensitivity Tests Mutation Neutralization Tests Phospholipids / metabolism RNA / metabolism Tumor Necrosis Factor-alpha / metabolism |
| IF | 2.476 |
| 引用数 | 16 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |