Reference - Detail
| RRC ID | 41515 |
|---|---|
| Author | Gansuvd B, Hagihara M, Yu Y, Inoue H, Ueda Y, Tsuchiya T, Masui A, Ando K, Nakamura Y, Munkhtuvshin N, Kato S, Thomas JM, Hotta T. |
| Title | Human umbilical cord blood NK T cells kill tumors by multiple cytotoxic mechanisms. |
| Journal | Hum Immunol |
| Abstract |
Natural killer (NK) T cells are restricted by CD1d and play an important role in the rejection of malignant tumors, but how kill these tumors is unclear. To investigate this, we cultured Valpha24+CD4+ NK T cells in human umbilical cord blood, which was enriched by immunomagnetic beads. In short-term (4 h) cytotoxicity assays, the NK T cells could kill only those targets expressing CD1d. In longer cytotoxicity assays (20 h), however, the NK T cells were able to kill all the tumors, regardless of CD1d expression. When each of the perforin, Fas-FasL, and TNF-alpha cytotoxic mechanisms were blocked, it was apparent that perforin killing dominated in both the short- and long-term assays. In the short-term assay, perforin killing required that the targets expressed CD1d, but killing was more efficient if Fas was present because then the Fas-FasL mechanism was also used. Thus, cells that lacked Fas and CD1d and were not killed in the 4-h assay, were instead lysed in 20-h assay through a combination of perforin and TNF-alpha killing. NK T cells can kill tumor targets by perforin, Fas-FasL, and TNF-alpha mechanisms. TNF-alpha killing requires longer contact between effectors and targets, suggesting that TNF-alpha acts by enhancing perforin killing. |
| Volume | 63(3) |
| Pages | 164-75 |
| Published | 2002-3-1 |
| DOI | 10.1016/s0198-8859(01)00382-2 |
| PII | S0198885901003822 |
| PMID | 11872234 |
| MeSH | Antigens, CD1 / immunology* Antigens, CD1 / metabolism Antigens, CD1d CD4-Positive T-Lymphocytes / metabolism Cells, Cultured Cytokines / analysis Cytokines / immunology Cytotoxicity, Immunologic* Dendritic Cells / immunology Dendritic Cells / metabolism Fas Ligand Protein Female Fetal Blood / immunology* Humans Interferon-gamma / analysis Killer Cells, Natural / immunology* Killer Cells, Natural / metabolism Lymphocyte Culture Test, Mixed Membrane Glycoproteins / immunology* Membrane Glycoproteins / metabolism Neoplasms / immunology* Neoplasms / metabolism Perforin Pore Forming Cytotoxic Proteins Tumor Necrosis Factor-alpha / immunology |
| IF | 2.412 |
| Times Cited | 26 |
| WOS Category | IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | K562(RCB0027) Jurkat(RCB0806) MOLT-4(RCB0206) |