Reference - Detail
| RRC ID | 41551 |
|---|---|
| Author | Narita N, Noda I, Ohtsubo T, Fujieda S, Tokuriki M, Saito T, Saito H. |
| Title | Analysis of heat-shock related gene expression in head-and-neck cancer using cDNA arrays. |
| Journal | Int J Radiat Oncol Biol Phys |
| Abstract |
PURPOSE:The aim of the present study was to identify genes regulated in the early response to heat-shock in human head-and-neck cancer cells using a cDNA array. METHODS AND MATERIALS:IMC-3 cells were heat-shocked at 44 degrees C for 30 min, then incubated for 6 h. After 6 h incubation, mRNAs were extracted. Early gene expressions in IMC-3 cells were analyzed using a cDNA array after heat-shock. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to identify the expressions of mRNA for confirming features by cDNA array analysis in several other cell lines (KB, T3M-1, SCC-TF) as well as in IMC-3 cells. RESULTS:Twenty-eight genes were found to be induced or upregulated by heat-shock in IMC-3 cells. These included genes involved in the apoptosis (e.g., CC3, caspase10), tumor invasion (e.g., CC3, TIMP-3), cell cycle checkpoint control (e.g., DP-1, CDC25A), signal transduction (e.g., MEK1) as well as genes associated with heat stress (e.g., Hsp70B', Hsp40). Gene expressions of CC3 and MEK1 were recognized to be induced by heat-shock in pharyngeal cancer cells (KB, T3M-1) and lingual cancer cells (SCC-TF). CONCLUSION:The observed alteration in gene expression may play a role in various biochemical pathways of cancer cells exposed to heat-shock. |
| Volume | 53(1) |
| Pages | 190-6 |
| Published | 2002-5-1 |
| DOI | 10.1016/s0360-3016(02)02727-x |
| PII | S036030160202727X |
| PMID | 12007959 |
| MeSH | Acetyltransferases* Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / therapy Fever* Gene Expression Profiling / methods* Head and Neck Neoplasms / genetics* Head and Neck Neoplasms / therapy Humans MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases / genetics Mitogen-Activated Protein Kinase Kinases / metabolism Oligonucleotide Array Sequence Analysis Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / genetics Transcription Factors / metabolism Tumor Cells, Cultured |
| IF | 5.859 |
| Times Cited | 27 |
| WOS Category | RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING ONCOLOGY |
| Resource | |
| Human and Animal Cells | T3M-1 SCCTF(RCB0442) |