論文 - 詳細
| RRC ID | 41579 |
|---|---|
| 著者 | Chong JM, Sakuma K, Sudo M, Osawa T, Ohara E, Uozaki H, Shibahara J, Kuroiwa K, Tominaga S, Hippo Y, Aburatani H, Funata N, Fukayama M. |
| タイトル | Interleukin-1beta expression in human gastric carcinoma with Epstein-Barr virus infection. |
| ジャーナル | J Virol |
| Abstract |
The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1beta (IL-1beta) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1beta mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1beta increased the cell growth of a GC cell line, TMK1. Thus, IL-1beta may act as an autocrine growth factor in EBVaGC. |
| 巻・号 | 76(13) |
| ページ | 6825-31 |
| 公開日 | 2002-7-1 |
| DOI | 10.1128/jvi.76.13.6825-6831.2002 |
| PMID | 12050395 |
| PMC | PMC136266 |
| MeSH | Adenocarcinoma / metabolism* Adenocarcinoma / virology Animals Epstein-Barr Virus Infections / metabolism* Herpesvirus 4, Human Humans Interleukin-1 / genetics Interleukin-1 / metabolism* Mice Mice, SCID Neoplasm Transplantation Oligonucleotide Array Sequence Analysis RNA, Messenger / genetics RNA, Messenger / metabolism Stomach Neoplasms / metabolism* Stomach Neoplasms / virology Tumor Cells, Cultured |
| IF | 4.501 |
| 引用数 | 40 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN7(RCB0999) MKN45(RCB1001) MKN74(RCB1002) |