| RRC ID |
416
|
| 著者 |
Mizuguchi S, Uyama T, Kitagawa H, Nomura KH, Dejima K, Gengyo-Ando K, Mitani S, Sugahara K, Nomura K.
|
| タイトル |
Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans.
|
| ジャーナル |
Nature
|
| Abstract |
Glycosaminoglycans such as heparan sulphate and chondroitin sulphate are extracellular sugar chains involved in intercellular signalling. Disruptions of genes encoding enzymes that mediate glycosaminoglycan biosynthesis have severe consequences in Drosophila and mice. Mutations in the Drosophila gene sugarless, which encodes a UDP-glucose dehydrogenase, impairs developmental signalling through the Wnt family member Wingless, and signalling by the fibroblast growth factor and Hedgehog pathways. Heparan sulphate is involved in these pathways, but little is known about the involvement of chondroitin. Undersulphated and oversulphated chondroitin sulphate chains have been implicated in other biological processes, however, including adhesion of erythrocytes infected with malaria parasite to human placenta and regulation of neural development. To investigate chondroitin functions, we cloned a chondroitin synthase homologue of Caenorhabditis elegans and depleted expression of its product by RNA-mediated interference and deletion mutagenesis. Here we report that blocking chondroitin synthesis results in cytokinesis defects in early embryogenesis. Reversion of cytokinesis is often observed in chondroitin-depleted embryos, and cell division eventually stops, resulting in early embryonic death. Our findings show that chondroitin is required for embryonic cytokinesis and cell division.
|
| 巻・号 |
423(6938)
|
| ページ |
443-8
|
| 公開日 |
2003-5-22
|
| DOI |
10.1038/nature01635
|
| PII |
nature01635
|
| PMID |
12761550
|
| MeSH |
Animals
Blotting, Western
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / embryology
Caenorhabditis elegans / enzymology
Caenorhabditis elegans / metabolism*
Carbohydrate Sequence
Cell Division
Chondroitin / deficiency
Chondroitin / metabolism*
Cloning, Molecular
Disaccharides / metabolism
Gene Deletion
Genes, Lethal / genetics
Glycosyltransferases / genetics
Glycosyltransferases / metabolism*
Molecular Sequence Data
N-Acetylgalactosaminyltransferases*
Phenotype
Proteoglycans / deficiency
Proteoglycans / metabolism*
RNA Interference
|
| IF |
42.779
|
| 引用数 |
181
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm546 |
