論文 - 詳細
| RRC ID | 41609 |
|---|---|
| 著者 | Kishikawa H, Miura S, Yoshida H, Hirokawa M, Nakamizo H, Higuchi H, Adachi M, Nakatsumi RC, Suzuki H, Saito H, Ishii H. |
| タイトル | Transmural pressure induces IL-6 secretion by intestinal epithelial cells. |
| ジャーナル | Clin Exp Immunol |
| Abstract |
Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80 mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 cells at 12 h. Under control conditions, IL-6 secretion was directed to the basolateral side, but after exposure to pressure IL-6 secretion was increased in both the apical and basolateral sides. A nuclear factor kappa B (NF-kappaB) decoy reversed completely the pressure-induced increase of IL-6 secretion by IEC-18 cells. Pressure treatment enhanced IL-6 mRNA expression in IECs within 6 h. Pressure loading significantly enhanced the activation of both NF-kappaB and NF-IL-6 from 1h in the nuclear protein of IEC-18 cells as assessed by the electrophoretic mobility shift assay using FITC-conjugated specific primers. Increased phosphorylation of I-kappa B was also demonstrated in the cytosol of IEC cells within 1h by Western blot analysis. These results suggest a possible role for pressure loading in immune modulation of the intestinal mucosa by the stimulation of IL-6 release from intestinal epithelial cells. |
| 巻・号 | 129(1) |
| ページ | 86-91 |
| 公開日 | 2002-7-1 |
| DOI | 10.1046/j.1365-2249.2002.01895.x |
| PII | 1895 |
| PMID | 12100026 |
| PMC | PMC1906408 |
| MeSH | Animals CCAAT-Enhancer-Binding Protein-beta / metabolism Cell Line / metabolism Cell Polarity DNA-Binding Proteins / metabolism Electrophoretic Mobility Shift Assay Epithelial Cells / metabolism Gene Expression Regulation / physiology* I-kappa B Proteins* Interleukin-6 / biosynthesis Interleukin-6 / genetics Interleukin-6 / metabolism* Intestinal Mucosa / metabolism* NF-KappaB Inhibitor alpha NF-kappa B / antagonists & inhibitors NF-kappa B / metabolism Phosphorylation Pressure* Protein Processing, Post-Translational RNA, Messenger / biosynthesis RNA, Messenger / genetics Rats Stress, Mechanical Time Factors Transcription, Genetic |
| IF | 3.532 |
| 引用数 | 11 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 7-TD-1(RCB1190) |