RRC ID 41609
著者 Kishikawa H, Miura S, Yoshida H, Hirokawa M, Nakamizo H, Higuchi H, Adachi M, Nakatsumi RC, Suzuki H, Saito H, Ishii H.
タイトル Transmural pressure induces IL-6 secretion by intestinal epithelial cells.
ジャーナル Clin Exp Immunol
Abstract Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80 mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 cells at 12 h. Under control conditions, IL-6 secretion was directed to the basolateral side, but after exposure to pressure IL-6 secretion was increased in both the apical and basolateral sides. A nuclear factor kappa B (NF-kappaB) decoy reversed completely the pressure-induced increase of IL-6 secretion by IEC-18 cells. Pressure treatment enhanced IL-6 mRNA expression in IECs within 6 h. Pressure loading significantly enhanced the activation of both NF-kappaB and NF-IL-6 from 1h in the nuclear protein of IEC-18 cells as assessed by the electrophoretic mobility shift assay using FITC-conjugated specific primers. Increased phosphorylation of I-kappa B was also demonstrated in the cytosol of IEC cells within 1h by Western blot analysis. These results suggest a possible role for pressure loading in immune modulation of the intestinal mucosa by the stimulation of IL-6 release from intestinal epithelial cells.
巻・号 129(1)
ページ 86-91
公開日 2002-7-1
DOI 10.1046/j.1365-2249.2002.01895.x
PII 1895
PMID 12100026
PMC PMC1906408
MeSH Animals CCAAT-Enhancer-Binding Protein-beta / metabolism Cell Line / metabolism Cell Polarity DNA-Binding Proteins / metabolism Electrophoretic Mobility Shift Assay Epithelial Cells / metabolism Gene Expression Regulation / physiology* I-kappa B Proteins* Interleukin-6 / biosynthesis Interleukin-6 / genetics Interleukin-6 / metabolism* Intestinal Mucosa / metabolism* NF-KappaB Inhibitor alpha NF-kappa B / antagonists & inhibitors NF-kappa B / metabolism Phosphorylation Pressure* Protein Processing, Post-Translational RNA, Messenger / biosynthesis RNA, Messenger / genetics Rats Stress, Mechanical Time Factors Transcription, Genetic
IF 3.532
引用数 11
WOS 分野 IMMUNOLOGY
リソース情報
ヒト・動物細胞 7-TD-1(RCB1190)