RRC ID 41616
Author Ichino N, Yamada K, Nishii K, Sawada H, Nagatsu T, Ishiguro H.
Title Increase of transcriptional levels of egr-1 and nur77 genes due to both nicotine treatment and withdrawal in pheochromocytoma cells.
Journal J Neural Transm (Vienna)
Abstract The influence of nicotine on the expression of egr-1 and nur77 genes by nicotine treatment and withdrawal was assessed using PC12 cells. Nicotine treatment significantly increased the amount of mRNA for egr-1 and nur77 genes at 0.5 h post-nicotine treatment in the PC12 cells. In addition, nicotine withdrawal also elevated transcriptional levels of egr-1 and nur77 genes in Northern blot analyses. Nicotine treatment (200 microM) was also found to significantly increase expressional levels of Egr-1 and Nur77 proteins at 0.5 h post-nicotine treatment. In contrast, Egr-1 and Nur77 protein levels were dramatically decreased by nicotine withdrawal. These results suggest that expressional levels of Egr-1 and Nur77 proteins in neural cells may affect the transcriptional activity of late-response genes after nicotine withdrawal.
Volume 109(7-8)
Pages 1015-22
Published 2002-7-1
DOI 10.1007/s007020200084
PMID 12111438
MeSH Animals DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Early Growth Response Protein 1 Immediate-Early Proteins* Nicotine / adverse effects* Nicotine / pharmacology Nuclear Receptor Subfamily 4, Group A, Member 1 PC12 Cells / drug effects PC12 Cells / physiology* RNA, Messenger / metabolism Rats Receptors, Cytoplasmic and Nuclear Receptors, Steroid Substance Withdrawal Syndrome / genetics* Substance Withdrawal Syndrome / metabolism Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic / drug effects*
IF 3.505
Times Cited 12
Human and Animal Cells