論文 - 詳細
| RRC ID | 41626 |
|---|---|
| 著者 | Tanaka J, Koshimura K, Murakami Y, Kato Y. |
| タイトル | Possible involvement of tetrahydrobiopterin in the trophic effect of insulin-like growth factor-1 on rat pheochromocytoma-12 (PC12) cells. |
| ジャーナル | Neurosci Lett |
| Abstract |
Tetrahydrobiopterin (BH(4)) has a trophic effect on pheochromocytoma-12 (PC12) cells such as insulin-like growth factor-1 (IGF-1). We investigated involvement of BH(4) in the trophic effect of IGF-1 on PC12 cells. IGF-1 (10-300 ng/ml) increased cellular BH(4) content in a dose-related manner. Cellular BH(4) content increased after 6-36 h incubation with IGF-1. IGF-1-induced increase in the cellular BH(4) content was blunted by 0.3 mM 2,4-diamino-6-hydroxypyrimidine (DAHP), an inhibitor for BH(4) synthesis. IGF-1 protected PC12 cells from the cell death induced by depletion of serum and nerve growth factor, which was attenuated by DAHP. The effects of IGF-1 on the cellular BH(4) content and cell viability were eliminated by 0.2 microM wortmannin. These results suggest that BH(4) is involved in the trophic effect of IGF-1 on PC12 cells and that the effect of IGF-1 on BH(4) synthesis is mediated by phosphatidylinositol 3-kinase. |
| 巻・号 | 328(2) |
| ページ | 201-3 |
| 公開日 | 2002-8-9 |
| DOI | 10.1016/s0304-3940(02)00497-4 |
| PII | S0304394002004974 |
| PMID | 12133588 |
| MeSH | Androstadienes / pharmacology Animals Biopterin / analogs & derivatives* Biopterin / antagonists & inhibitors Biopterin / biosynthesis* Brain / growth & development* Brain / metabolism* Cell Differentiation / drug effects Cell Differentiation / physiology* Cell Survival / drug effects Cell Survival / physiology Culture Media, Serum-Free / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Hypoxanthines / pharmacology Insulin-Like Growth Factor I / metabolism* Insulin-Like Growth Factor I / pharmacology Nerve Growth Factor / deficiency Nerve Growth Factors / metabolism* Nerve Growth Factors / pharmacology Neurons / drug effects Neurons / metabolism* PC12 Cells Phosphatidylinositol 3-Kinases / drug effects Phosphatidylinositol 3-Kinases / metabolism Rats Wortmannin |
| IF | 2.274 |
| 引用数 | 7 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| ヒト・動物細胞 | |