Reference - Detail
| RRC ID | 41650 |
|---|---|
| Author | Wichterle H, Lieberam I, Porter JA, Jessell TM. |
| Title | Directed differentiation of embryonic stem cells into motor neurons. |
| Journal | Cell |
| Abstract |
Inductive signals and transcription factors involved in motor neuron generation have been identified, raising the question of whether these developmental insights can be used to direct stem cells to a motor neuron fate. We show that developmentally relevant signaling factors can induce mouse embryonic stem (ES) cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo. ES cell-derived motor neurons can populate the embryonic spinal cord, extend axons, and form synapses with target muscles. Thus, inductive signals involved in normal pathways of neurogenesis can direct ES cells to form specific classes of CNS neurons. |
| Volume | 110(3) |
| Pages | 385-97 |
| Published | 2002-8-9 |
| DOI | 10.1016/s0092-8674(02)00835-8 |
| PII | S0092867402008358 |
| PMID | 12176325 |
| MeSH | Animals Body Patterning / drug effects Body Patterning / physiology Cell Communication / physiology Cell Differentiation / drug effects Cell Differentiation / physiology* Cells, Cultured Chick Embryo Embryonic Induction / drug effects Embryonic Induction / physiology* Graft Survival / physiology Green Fluorescent Proteins Hedgehog Proteins Homeodomain Proteins / metabolism Indicators and Reagents Luminescent Proteins Mice Mice, Transgenic Motor Neurons / cytology Motor Neurons / drug effects Motor Neurons / metabolism* Signal Transduction / drug effects Signal Transduction / physiology* Spheroids, Cellular / cytology Spheroids, Cellular / drug effects Spheroids, Cellular / metabolism Spinal Cord / cytology Spinal Cord / embryology* Spinal Cord / metabolism Stem Cells / cytology Stem Cells / drug effects Stem Cells / metabolism* Trans-Activators / agonists Trans-Activators / metabolism Transcription Factors / metabolism Tretinoin / pharmacology |
| IF | 38.637 |
| Times Cited | 1194 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-G2/PA6(RCB1127) |