Reference - Detail
|Author||Tanaka K, Fujita N, Higashi Y, Ogawa N.|
|Title||Neuroprotective and antioxidant properties of FKBP-binding immunophilin ligands are independent on the FKBP12 pathway in human cells.|
We focused on immunophilin isoforms in order to clarify the neuroimmunophilins which were identified as targets for the immunophilin ligands to elicit a neuroprotective effect. Although the expressions of five FK506-binding protein (FKBP) mRNAs were detected in both SH-SY5Y (human neuroblastoma) and U251 (human glioma) cell lysates, the FKBP12 mRNA expression was detected in only the SH-SY5Y cells, and not the U251 cells. However, we found that the SH-SY5Y and the U251 cells were equipotent in the intensity of cellular protection of FK506 (an immunosuppressive immunophilin ligand) and GPI1046 (a non-immunosuppressive FK506 analog), indicating that the protective effect and glutathione activation of FK506 and GPI1046 had little need to bind FKBP12. Therefore, we conclude that the neuroprotective and antioxidant properties of immunophilin ligands are independent on the FKBP12 pathway.
|MeSH||Actins / genetics Actins / metabolism Antioxidants / pharmacology Cell Survival / drug effects Colorimetry Dimethyl Sulfoxide / pharmacology Glutathione / drug effects Glutathione / metabolism Humans Hydrogen Peroxide / pharmacology* Immunosuppressive Agents / pharmacology* Neuroprotective Agents / pharmacology Peptidylprolyl Isomerase* Pyrrolidines / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Tacrolimus / pharmacology* Tacrolimus Binding Protein 1A / drug effects Tacrolimus Binding Protein 1A / genetics Tacrolimus Binding Protein 1A / physiology* Tacrolimus Binding Proteins / genetics* Tacrolimus Binding Proteins / metabolism Tubulin / genetics Tubulin / metabolism Tumor Cells, Cultured|
|Human and Animal Cells|